Time course of lipid‐laden pulmonary macrophages with acute and recurrent milk aspiration in rabbits

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

High levels of lipid‐laden macrophages (LLM) in bronchial washings have been associated with food aspiration. We studied the time course of appearance and clearance of LLM in rabbits undergoing either a single milk instillation, five weekly milk instillations or saline (control) instillations into the airways. Cells were obtained by bronchoalveolar lavage of intubated rabbits at uniform time intervals following the single or the last of five milk/saline instillations. LLM semi‐quantitative indexes were derived using oil‐red‐O staining. Significantly elevated indexes were found in both milk groups 6 hr after milk instillation. In the single saline and milk instillation groups the indexes were not different beginning on the 4th day, and indexes from 8 of 9 rabbits had returned to baseline by the 6th day. However, indexes remained significantly elevated up to 17 days in the group receiving weekly milk instillations. Indexes from all rabbits in the repeat milk instillation group remained elevated for 12 days or longer. This group also developed increased numbers of binucleated macrophages. Quantitation of LLM in this model appears to be a sensitive indicator of recurrent lipid aspiration, these cells remaining in the airways for several days after the last aspiration event.

Original languageGerman
Pages (from-to)95-98
Number of pages4
JournalPediatric Pulmonology
Volume12
Issue number2
DOIs
StatePublished - Feb 1992

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Alveolar Macrophages
Milk
Rabbits
Lipids
Macrophages
Bronchoalveolar Lavage
Staining and Labeling
Food

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pulmonary and Respiratory Medicine

Cite this

Time course of lipid‐laden pulmonary macrophages with acute and recurrent milk aspiration in rabbits. / Colombo, John Louis; Hallberg, T. K.; Sammut, Paul Henry.

In: Pediatric Pulmonology, Vol. 12, No. 2, 02.1992, p. 95-98.

Research output: Contribution to journalArticle

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