Time course of fiber outgrowth from fetal anterior hypothalamic heterografts

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fetal anterior hypothalamic (AH) heterografts can restore circadian rhythmicity to animals rendered arhythmic following ablation of the suprachiasmatic nucleus (SCN). Behavioral restoration of circadian activity typically begins between two and six weeks post-implantation. The time course of fiber outgrowth from fetal AH heterografts was examined to determine whether neuronal outgrowth from the implants precedes the typically observed effects of such implants upon circadian behavior. Fetal mouse or rat AH tissue containing the SCN was implanted into the third ventricle of SCN-lesioned hamsters. Using species-specific monoclonal antibodies generated against mouse or rat neuronal elements, fiber outgrowth into the host hypothalamus was examined at 2, 4, 7, 14, 30 and 45 days after implantation. Fibers were observed to have emerged from the implant at the earliest time point examined. Four days after surgery, individual fibers had extended up to 0.6 mm into the host neuropil. By 14 days post-implantation, outgrowth from the implant had formed a dense fiber plexus in the host hypothalamus. This observation demonstrates that neuronal integration of the implant with the host brain begins within 48 hours of implantation, and is extensively established well before a restoration of rhythmicity is typically observed. Thus, on the basis of the time course of fiber outgrowth, it is clear that neuronal contact between graft and host may mediate the observed restoration of circadian rhythmicity.

Original languageEnglish (US)
Pages (from-to)212-219
Number of pages8
JournalBrain Research
Volume614
Issue number1-2
DOIs
StatePublished - Jun 18 1993

Fingerprint

Suprachiasmatic Nucleus
Periodicity
Heterografts
Hypothalamus
Third Ventricle
Neuropil
Ambulatory Surgical Procedures
Cricetinae
Monoclonal Antibodies
Transplants
Brain

Keywords

  • Fetal hypothalamic graft
  • Fiber outgrowth
  • Heterograft
  • Neural transplantation
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Time course of fiber outgrowth from fetal anterior hypothalamic heterografts. / Sollars, Patricia J.; Pickard, Gary E.

In: Brain Research, Vol. 614, No. 1-2, 18.06.1993, p. 212-219.

Research output: Contribution to journalArticle

@article{d15ac20882f2468dbdd925970713de29,
title = "Time course of fiber outgrowth from fetal anterior hypothalamic heterografts",
abstract = "Fetal anterior hypothalamic (AH) heterografts can restore circadian rhythmicity to animals rendered arhythmic following ablation of the suprachiasmatic nucleus (SCN). Behavioral restoration of circadian activity typically begins between two and six weeks post-implantation. The time course of fiber outgrowth from fetal AH heterografts was examined to determine whether neuronal outgrowth from the implants precedes the typically observed effects of such implants upon circadian behavior. Fetal mouse or rat AH tissue containing the SCN was implanted into the third ventricle of SCN-lesioned hamsters. Using species-specific monoclonal antibodies generated against mouse or rat neuronal elements, fiber outgrowth into the host hypothalamus was examined at 2, 4, 7, 14, 30 and 45 days after implantation. Fibers were observed to have emerged from the implant at the earliest time point examined. Four days after surgery, individual fibers had extended up to 0.6 mm into the host neuropil. By 14 days post-implantation, outgrowth from the implant had formed a dense fiber plexus in the host hypothalamus. This observation demonstrates that neuronal integration of the implant with the host brain begins within 48 hours of implantation, and is extensively established well before a restoration of rhythmicity is typically observed. Thus, on the basis of the time course of fiber outgrowth, it is clear that neuronal contact between graft and host may mediate the observed restoration of circadian rhythmicity.",
keywords = "Fetal hypothalamic graft, Fiber outgrowth, Heterograft, Neural transplantation, Suprachiasmatic nucleus",
author = "Sollars, {Patricia J.} and Pickard, {Gary E.}",
year = "1993",
month = "6",
day = "18",
doi = "10.1016/0006-8993(93)91037-S",
language = "English (US)",
volume = "614",
pages = "212--219",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Time course of fiber outgrowth from fetal anterior hypothalamic heterografts

AU - Sollars, Patricia J.

AU - Pickard, Gary E.

PY - 1993/6/18

Y1 - 1993/6/18

N2 - Fetal anterior hypothalamic (AH) heterografts can restore circadian rhythmicity to animals rendered arhythmic following ablation of the suprachiasmatic nucleus (SCN). Behavioral restoration of circadian activity typically begins between two and six weeks post-implantation. The time course of fiber outgrowth from fetal AH heterografts was examined to determine whether neuronal outgrowth from the implants precedes the typically observed effects of such implants upon circadian behavior. Fetal mouse or rat AH tissue containing the SCN was implanted into the third ventricle of SCN-lesioned hamsters. Using species-specific monoclonal antibodies generated against mouse or rat neuronal elements, fiber outgrowth into the host hypothalamus was examined at 2, 4, 7, 14, 30 and 45 days after implantation. Fibers were observed to have emerged from the implant at the earliest time point examined. Four days after surgery, individual fibers had extended up to 0.6 mm into the host neuropil. By 14 days post-implantation, outgrowth from the implant had formed a dense fiber plexus in the host hypothalamus. This observation demonstrates that neuronal integration of the implant with the host brain begins within 48 hours of implantation, and is extensively established well before a restoration of rhythmicity is typically observed. Thus, on the basis of the time course of fiber outgrowth, it is clear that neuronal contact between graft and host may mediate the observed restoration of circadian rhythmicity.

AB - Fetal anterior hypothalamic (AH) heterografts can restore circadian rhythmicity to animals rendered arhythmic following ablation of the suprachiasmatic nucleus (SCN). Behavioral restoration of circadian activity typically begins between two and six weeks post-implantation. The time course of fiber outgrowth from fetal AH heterografts was examined to determine whether neuronal outgrowth from the implants precedes the typically observed effects of such implants upon circadian behavior. Fetal mouse or rat AH tissue containing the SCN was implanted into the third ventricle of SCN-lesioned hamsters. Using species-specific monoclonal antibodies generated against mouse or rat neuronal elements, fiber outgrowth into the host hypothalamus was examined at 2, 4, 7, 14, 30 and 45 days after implantation. Fibers were observed to have emerged from the implant at the earliest time point examined. Four days after surgery, individual fibers had extended up to 0.6 mm into the host neuropil. By 14 days post-implantation, outgrowth from the implant had formed a dense fiber plexus in the host hypothalamus. This observation demonstrates that neuronal integration of the implant with the host brain begins within 48 hours of implantation, and is extensively established well before a restoration of rhythmicity is typically observed. Thus, on the basis of the time course of fiber outgrowth, it is clear that neuronal contact between graft and host may mediate the observed restoration of circadian rhythmicity.

KW - Fetal hypothalamic graft

KW - Fiber outgrowth

KW - Heterograft

KW - Neural transplantation

KW - Suprachiasmatic nucleus

UR - http://www.scopus.com/inward/record.url?scp=0027281095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027281095&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(93)91037-S

DO - 10.1016/0006-8993(93)91037-S

M3 - Article

C2 - 8348314

AN - SCOPUS:0027281095

VL - 614

SP - 212

EP - 219

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -