Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice

Jennifer Nicholas, Joachim G. Voss, Joyce Tsuji, Nadia D. Fulkerson, Julia Soulakova, Barbara St Pierre Schneider

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Innate pro-inflammatory processes, such as chemokine signaling and leukocyte infiltration, predominate during the first 48 h after an acute skeletal muscle injury. However, the time course of chemokine expression and its relationship to leukocyte infiltration after acute muscle injury within this early post-injury time period has not been investigated. In this study, 46 anesthetized female C57BL/6NHsd mice underwent a closed crush injury of the gastrocnemius muscle and were euthanized 4, 8, 24 and 48 h post-injury. Microarray analysis found 14 chemokine genes to be up-regulated during this period, 12 of which are involved in macrophage or neutrophil chemotaxis, with up-regulation peaking at either 8 or 48 h. RT-PCR analysis on select chemokines confirmed the microarray activation pattern. Neutrophil infiltration patterns mirrored the time course of neutrophil-related chemokines with Gr-1-, 1A8- and 7/4-positive neutrophils infiltrating the muscle 4 h after injury, decreasing at 48 h. Conversely, gene expression and relative quantification levels of macrophage-related chemokines Ccl2 and Ccl7 peaked at 8 h, preceding the infiltration of CD68- and F4/80-positive macrophages, and protein expression of Ccl2 in the muscle. The up-regulation of other macrophage-related chemokines and their receptors peaked at 48 h post-injury.

Original languageEnglish (US)
Pages (from-to)266-274
Number of pages9
JournalInnate Immunity
Volume21
Issue number3
DOIs
StatePublished - Apr 15 2015

Fingerprint

Chemokines
Skeletal Muscle
Leukocytes
Wounds and Injuries
Macrophages
Neutrophils
Muscles
Up-Regulation
Neutrophil Infiltration
Chemokine Receptors
Chemotaxis
Microarray Analysis
Inbred C57BL Mouse
Gene Expression
Polymerase Chain Reaction
Genes
Proteins

Keywords

  • Inflammation
  • macrophage
  • microarray
  • muscle recovery
  • neutrophil

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

Cite this

Nicholas, J., Voss, J. G., Tsuji, J., Fulkerson, N. D., Soulakova, J., & Schneider, B. S. P. (2015). Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice. Innate Immunity, 21(3), 266-274. https://doi.org/10.1177/1753425914527326

Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice. / Nicholas, Jennifer; Voss, Joachim G.; Tsuji, Joyce; Fulkerson, Nadia D.; Soulakova, Julia; Schneider, Barbara St Pierre.

In: Innate Immunity, Vol. 21, No. 3, 15.04.2015, p. 266-274.

Research output: Contribution to journalArticle

Nicholas, J, Voss, JG, Tsuji, J, Fulkerson, ND, Soulakova, J & Schneider, BSP 2015, 'Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice', Innate Immunity, vol. 21, no. 3, pp. 266-274. https://doi.org/10.1177/1753425914527326
Nicholas, Jennifer ; Voss, Joachim G. ; Tsuji, Joyce ; Fulkerson, Nadia D. ; Soulakova, Julia ; Schneider, Barbara St Pierre. / Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice. In: Innate Immunity. 2015 ; Vol. 21, No. 3. pp. 266-274.
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