Three-dimensional printed trileaflet valve conduits using biological hydrogels and human valve interstitial cells

B. Duan, E. Kapetanovic, L. A. Hockaday, J. T. Butcher

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Tissue engineering has great potential to provide a functional de novo living valve replacement, capable of integration with host tissue and growth. Among various valve conduit fabrication techniques, three-dimensional (3-D) bioprinting enables deposition of cells and hydrogels into 3-D constructs with anatomical geometry and heterogeneous mechanical properties. Successful translation of this approach, however, is constrained by the dearth of printable and biocompatible hydrogel materials. Furthermore, it is not known how human valve cells respond to these printed environments. In this study, 3-D printable formulations of hybrid hydrogels are developed, based on methacrylated hyaluronic acid (Me-HA) and methacrylated gelatin (Me-Gel), and used to bioprint heart valve conduits containing encapsulated human aortic valvular interstitial cells (HAVIC). Increasing Me-Gel concentration resulted in lower stiffness and higher viscosity, facilitated cell spreading, and better maintained HAVIC fibroblastic phenotype. Bioprinting accuracy was dependent upon the relative concentrations of Me-Gel and Me-HA, but when optimized enabled the fabrication of a trileaflet valve shape accurate to the original design. HAVIC encapsulated within bioprinted heart valves maintained high viability, and remodeled the initial matrix by depositing collagen and glyosaminoglycans. These findings represent the first rational design of bioprinted trileaflet valve hydrogels that regulate encapsulated human VIC behavior. The use of anatomically accurate living valve scaffolds through bioprinting may accelerate understanding of physiological valve cell interactions and progress towards de novo living valve replacements.

Original languageEnglish (US)
Pages (from-to)1836-1846
Number of pages11
JournalActa Biomaterialia
Volume10
Issue number5
DOIs
StatePublished - May 2014

Fingerprint

Hydrogels
Gelatin
Bioprinting
Hyaluronic Acid
Hyaluronic acid
Fabrication
Hydrogel
Heart Valves
Bioelectric potentials
Tissue engineering
Scaffolds
Collagen
Stiffness
Viscosity
Tissue
Mechanical properties
Geometry
Biocompatible Materials
Tissue Engineering
Cell Communication

Keywords

  • Extracellular matrix
  • Microenvironment
  • Rapid prototyping
  • Remodeling
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Three-dimensional printed trileaflet valve conduits using biological hydrogels and human valve interstitial cells. / Duan, B.; Kapetanovic, E.; Hockaday, L. A.; Butcher, J. T.

In: Acta Biomaterialia, Vol. 10, No. 5, 05.2014, p. 1836-1846.

Research output: Contribution to journalArticle

Duan, B. ; Kapetanovic, E. ; Hockaday, L. A. ; Butcher, J. T. / Three-dimensional printed trileaflet valve conduits using biological hydrogels and human valve interstitial cells. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 5. pp. 1836-1846.
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