Three-dimensional nanofiber scaffolds with arrayed holes for engineering skin tissue constructs

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7 Citations (Scopus)

Abstract

Three-dimensional (3D) scaffolds composed of poly(ϵ-caprolactone) and gelatin nanofibers were fabricated by a combination of electrospinning and modified gas-foaming. Arrayed holes throughout the scaffold were created using a punch under cryo conditions. The crosslinking with glutaraldehyde vapor improved the water stability of the scaffolds. Cell spheroids of green fluorescent protein-labeled human dermal fibroblasts were prepared and seeded into the holes. It was found that the fibroblasts adhered well on the surface of nanofibers and migrated into the scaffolds due to the porous structures. The 3D nanofiber scaffolds may hold great potential for engineering tissue constructs for various applications.

Original languageEnglish (US)
Pages (from-to)361-366
Number of pages6
JournalMRS Communications
Volume7
Issue number3
DOIs
StatePublished - Sep 1 2017

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Scaffolds (biology)
Nanofibers
Skin
Fibroblasts
Tissue
Scaffolds
Gas foaming
Bioelectric potentials
Electrospinning
Glutaral
Gelatin
Green Fluorescent Proteins
Tissue engineering
Crosslinking
Vapors
Water
Proteins

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

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abstract = "Three-dimensional (3D) scaffolds composed of poly(ϵ-caprolactone) and gelatin nanofibers were fabricated by a combination of electrospinning and modified gas-foaming. Arrayed holes throughout the scaffold were created using a punch under cryo conditions. The crosslinking with glutaraldehyde vapor improved the water stability of the scaffolds. Cell spheroids of green fluorescent protein-labeled human dermal fibroblasts were prepared and seeded into the holes. It was found that the fibroblasts adhered well on the surface of nanofibers and migrated into the scaffolds due to the porous structures. The 3D nanofiber scaffolds may hold great potential for engineering tissue constructs for various applications.",
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