Thin-layer chromatographic screening methods for histamine in tuna fish

Ellen R. Lieber, Steve L. Taylor

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Twelve solvent systems were tested for their ability to separate histamine and histidine on a variety of thin-layer coatings. The best solvent-adsorbent systems were: chloroform-methanol-ammonia (2:2:1), methanol-ammonia (20:1), acetone-ammonia (95:5), and double development with (a) n-butanol-acetone-water (2:2:1) and (b) chloroform-methanol-ammonia (12:7:1), all on siliga-gel layers. Ninhydrin was used as the visualization reagent. These four systems were then evaluated for their potential use as rapid screening procedures in the detection of possibly deleterious levels of histamine in tuna fish. Successful separation of histamine from the other ninhydrin-positive components of methanolic tuna fish extracts was achieved with all four systems. A sample from a lot of tuna implicated in human illness was found to have a histamine level considerably higher than tuna purchased from a local retail outlet or an extract spiked to a histamine level considered to be a threshold value for toxicity symptoms. The methanol-ammonia (20:1) and chloroform-methanol-ammonia (2:2:1) systems, used with silica-gel plates, are the most promising for rapid preliminary screening of tuna fish extracts for histamine.

Original languageEnglish (US)
Pages (from-to)143-152
Number of pages10
JournalJournal of Chromatography A
Volume153
Issue number1
DOIs
StatePublished - Jun 1 1978

Fingerprint

Tuna
Ammonia
Fish
Histamine
Screening
Fishes
Methanol
Chloroform
Ninhydrin
Acetone
1-Butanol
Silica Gel
Histidine
Adsorbents
Toxicity
Visualization
Gels
Coatings
Water

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Thin-layer chromatographic screening methods for histamine in tuna fish. / Lieber, Ellen R.; Taylor, Steve L.

In: Journal of Chromatography A, Vol. 153, No. 1, 01.06.1978, p. 143-152.

Research output: Contribution to journalArticle

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