Therapeutic effects of Nigella sativa on chronic HAART- Induced hyperinsulinemia in rats

Surabhi Chandra, Subramanyam N. Murthy, Debasis Mondal, Krishna C. Agrawal

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Prolonged use of highly active antiretroviral therapy (HAART) is associated with insulin resistance in HIV-1- positive patients. Small animal models that recapitulate the long-term effects of HAART may facilitate the identification of therapeutic agents to suppress these side effects. We investigated the protective effects of black seed oil (BSO) from Nigella sativa in Sprague-Dawley rats treated with a daily HAART regimen for 7 months. The antiretroviral drugs, consisting of nelfinavir (200 mg/kg), zidovudine (50 mg/kg), and efavirenz (20 mg/kg), were mixed with diet with or without BSO (400 μL/kg) supplementation. Significant increases in insulin and C- peptide levels were observed in HAART- treated groups, and concomitant BSO treatment reduced this hyperinsulinemia. Interestingly, HAART- treated rats showed reduced size of pancreatic islets that was not seen in BSO- exposed rats. In vitro studies showed that nelfinavir, alone and in combination with HAART, induced oxidative stress and decreased glucose-induced insulin production in INS-1 cells. Suppressed insulin production was restored in cells coexposed to either BSO or thymoquinone. Our findings demonstrated that chronic HAART may increase serum insulin levels by dysregulating both insulin production by β cells and insulin action at the periphery. These deleterious effects may be prevented by dietary supplementation with BSO.

Original languageEnglish (US)
Pages (from-to)300-309
Number of pages10
JournalCanadian journal of physiology and pharmacology
Volume87
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Nigella sativa
Highly Active Antiretroviral Therapy
Hyperinsulinism
Therapeutic Uses
Seeds
Insulin
Oils
Nelfinavir
efavirenz
Zidovudine
C-Peptide
Dietary Supplements
Islets of Langerhans
Sprague Dawley Rats
Insulin Resistance
HIV-1
Oxidative Stress
Animal Models
Diet
Glucose

Keywords

  • Black seed oil
  • Haart drugs
  • Hyperinsulinemia
  • Ins-1 cells
  • Long-term exposure
  • Pancreatic islets
  • Rats
  • Therapeutics

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Therapeutic effects of Nigella sativa on chronic HAART- Induced hyperinsulinemia in rats. / Chandra, Surabhi; Murthy, Subramanyam N.; Mondal, Debasis; Agrawal, Krishna C.

In: Canadian journal of physiology and pharmacology, Vol. 87, No. 4, 01.04.2009, p. 300-309.

Research output: Contribution to journalArticle

Chandra, Surabhi ; Murthy, Subramanyam N. ; Mondal, Debasis ; Agrawal, Krishna C. / Therapeutic effects of Nigella sativa on chronic HAART- Induced hyperinsulinemia in rats. In: Canadian journal of physiology and pharmacology. 2009 ; Vol. 87, No. 4. pp. 300-309.
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