Theranostic fluorescent silica encapsulated magnetic nanoassemblies for in vitro MRI imaging and hyperthermia

Sunil Kumar, Amita Daverey, Vahid Khalilzad-Sharghi, Niroj K. Sahu, Srivatsan S Kidambi, Shadi F. Othman, Dhirendra Bahadur

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This article reports the synthesis of manganese ferrite nano-assemblies (MNAs) encapsulated with fluorescent silica shell and demonstrates their applicability for magnetic hyperthermia, optical and T2 contrast MRI imaging with HeLa cancer cells. The MNAs were encapsulated by a double layer of silica shell through a two-step sol-gel process. The inner silica shell contains rhodamine-B isothiocyanate (RITC) dye, whereas the outer silica layer is without RITC-dye, helps to prevent photo-bleaching and increase photo-luminance. MNAs@Dye-SiO2@SiO2 exhibited a high magnetization of 90.43 emu g-1 with a remarkably high r2 value of 598 ± 2 mM-1 s-1 (Mn + Fe). The cellular uptake of MNAs@Dye-SiO2@SiO2 was observed by the presence of fluorescent red granulated spots in the cytoplasm of HeLa cells, confirming its efficacy for optical imaging. High transverse relaxivities r2 (darkening effect) were observed in HeLa cells incubated with MNAs@Dye-SiO2@SiO2 in comparison to HeLa cells without particles. An 80-85% cell death was achieved on induction of magnetic hyperthermia with HeLa cells at the lowest Hf factor value (3.3 × 109 A m-1 s-1). Our results show MNAs@Dye-SiO2@SiO2 as a novel multifunctional theranostic nanoprobe, which can realize its applicability for diagnostic and real time monitoring the efficacy of ongoing cancer therapy.

Original languageEnglish (US)
Pages (from-to)53180-53188
Number of pages9
JournalRSC Advances
Volume5
Issue number66
DOIs
StatePublished - Jan 1 2015

Fingerprint

Silicon Dioxide
Magnetic resonance imaging
Manganese
Ferrite
Coloring Agents
Dyes
Silica
Imaging techniques
Nanoprobes
Photobleaching
Cell death
Sol-gel process
manganese ferrite
Luminance
Magnetization
Cells
Monitoring
rhodamine isothiocyanate

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Kumar, S., Daverey, A., Khalilzad-Sharghi, V., Sahu, N. K., Kidambi, S. S., Othman, S. F., & Bahadur, D. (2015). Theranostic fluorescent silica encapsulated magnetic nanoassemblies for in vitro MRI imaging and hyperthermia. RSC Advances, 5(66), 53180-53188. https://doi.org/10.1039/c5ra07632c

Theranostic fluorescent silica encapsulated magnetic nanoassemblies for in vitro MRI imaging and hyperthermia. / Kumar, Sunil; Daverey, Amita; Khalilzad-Sharghi, Vahid; Sahu, Niroj K.; Kidambi, Srivatsan S; Othman, Shadi F.; Bahadur, Dhirendra.

In: RSC Advances, Vol. 5, No. 66, 01.01.2015, p. 53180-53188.

Research output: Contribution to journalArticle

Kumar, S, Daverey, A, Khalilzad-Sharghi, V, Sahu, NK, Kidambi, SS, Othman, SF & Bahadur, D 2015, 'Theranostic fluorescent silica encapsulated magnetic nanoassemblies for in vitro MRI imaging and hyperthermia', RSC Advances, vol. 5, no. 66, pp. 53180-53188. https://doi.org/10.1039/c5ra07632c
Kumar, Sunil ; Daverey, Amita ; Khalilzad-Sharghi, Vahid ; Sahu, Niroj K. ; Kidambi, Srivatsan S ; Othman, Shadi F. ; Bahadur, Dhirendra. / Theranostic fluorescent silica encapsulated magnetic nanoassemblies for in vitro MRI imaging and hyperthermia. In: RSC Advances. 2015 ; Vol. 5, No. 66. pp. 53180-53188.
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