The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells

Nina K. Heinzinger, Michael I. Bukrinsky, Sheryl A. Haggerty, Anna M. Ragland, Vineet Kewalramani, May Ann Lee, Howard Eliot Gendelman, Lee Ratner, Mario Stevenson, Michael Emerman

Research output: Contribution to journalArticle

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Abstract

The replication of human immunodeficiency virus type 1 (HIV-1) in nondividing host cells such as those of macrophage lineage is an important feature of AIDS pathogenesis. The pattern of HIV-1 replication is dictated, in part, by the nucleophilic property of the viral gag matrix (MA) protein, a component of the viral preintegration complex that facilitates nuclear localization of viral nucleic acids in the absence of mitosis. We now identify the accessory viral protein Vpr, as a second nucleophilic component that influences nuclear localization of viral nucleic acids in nondividing cells. Reverse transcription and nuclear localization of viral nucleic acids following infection of cells by viruses lacking Vpr or viruses containing mutations in a gag MA nuclear localization sequence were indistinguishable from the pattern observed in cells infected by wild-type HIV-1. These viruses retained the ability to replicate in both dividing and nondividing host cells including monocyte-derived macrophages. In contrast, introduction of both gag MA and Vpr mutations in HIV-1 attenuated nuclear localization of viral nucleic acids in nondividing cells and virus replication in monocyte-derived macrophages. These studies demonstrate redundant nucleophilic determinants of HIV-1 that independently permit nuclear localization of viral nucleic acids and virus replication in nondividing cells such as monocyte-derived macrophages. In addition, these studies provide a defined function for an accessory gene product of HIV-1.

Original languageEnglish (US)
Pages (from-to)7311-7315
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number15
DOIs
StatePublished - Jul 19 1994

Fingerprint

vpr Gene Products
Nucleic Acids
HIV-1
Virus Replication
Macrophages
Viral Regulatory and Accessory Proteins
Viral Matrix Proteins
Viruses
gag Gene Products
Nuclear Matrix
Viral Structures
Mutation
Virus Diseases
Mitosis
Reverse Transcription
Acquired Immunodeficiency Syndrome

Keywords

  • AIDS
  • preintegration complex transport

ASJC Scopus subject areas

  • General

Cite this

The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells. / Heinzinger, Nina K.; Bukrinsky, Michael I.; Haggerty, Sheryl A.; Ragland, Anna M.; Kewalramani, Vineet; Lee, May Ann; Gendelman, Howard Eliot; Ratner, Lee; Stevenson, Mario; Emerman, Michael.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 15, 19.07.1994, p. 7311-7315.

Research output: Contribution to journalArticle

Heinzinger, Nina K. ; Bukrinsky, Michael I. ; Haggerty, Sheryl A. ; Ragland, Anna M. ; Kewalramani, Vineet ; Lee, May Ann ; Gendelman, Howard Eliot ; Ratner, Lee ; Stevenson, Mario ; Emerman, Michael. / The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 15. pp. 7311-7315.
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