The viral genome in experimental murine coxsackievirus B3 myocarditis

A Northern blotting anaylsis

Ikutaro Okada, Akira Matsumori, Chuichi Kawai, Junji Yodoi, Steven M Tracy

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The presence of viral genomes in the murine heart in experimental coxsackievirus B3 myocarditis was investigated by Northern blotting analysis. Four-week-old C3H He mice were inoculated with coxsackievirus B3. After sacrifice, the hearts were divided into 3 parts. Total RNA was extracted from one part of the heart. The other two parts were used for investigation of histopathology and of virus titer by plaque assay. The 32 P-labeled cDNA probe was derived from the 5′ end sequence of the coxsackievirus B3 genome. Northern blot autoradiograms of heart RNA were positive for viral RNA until day 7 and negative after day 10 and in control (uninfected) hearts. Positive autoradiograms always had the strongest signal at about 7.4 kilobase, corresponding to the size of the complete genome of the virus. The viral genomes were detected earlier than the appearance of the histopathologic changes in the murine heart. This type analysis of the viral genome in the murine myocardium may be useful in evaluating the effects of specific drugs on experimental viral myocarditis at the level of the viral genome.

Original languageEnglish (US)
Pages (from-to)999-1008
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume22
Issue number9
DOIs
StatePublished - Jan 1 1990

Fingerprint

Enterovirus
Viral Genome
Myocarditis
Northern Blotting
RNA
Genome Size
Inbred C3H Mouse
Viral RNA
Viral Load
Myocardium
Complementary DNA
Genome
Viruses
Pharmaceutical Preparations

Keywords

  • Coxsackievirus B3
  • Murine myocarditis
  • Northern blotting
  • Viral genome
  • cDNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

The viral genome in experimental murine coxsackievirus B3 myocarditis : A Northern blotting anaylsis. / Okada, Ikutaro; Matsumori, Akira; Kawai, Chuichi; Yodoi, Junji; Tracy, Steven M.

In: Journal of Molecular and Cellular Cardiology, Vol. 22, No. 9, 01.01.1990, p. 999-1008.

Research output: Contribution to journalArticle

Okada, Ikutaro ; Matsumori, Akira ; Kawai, Chuichi ; Yodoi, Junji ; Tracy, Steven M. / The viral genome in experimental murine coxsackievirus B3 myocarditis : A Northern blotting anaylsis. In: Journal of Molecular and Cellular Cardiology. 1990 ; Vol. 22, No. 9. pp. 999-1008.
@article{07ff946920244cd49b66db584c223513,
title = "The viral genome in experimental murine coxsackievirus B3 myocarditis: A Northern blotting anaylsis",
abstract = "The presence of viral genomes in the murine heart in experimental coxsackievirus B3 myocarditis was investigated by Northern blotting analysis. Four-week-old C3H He mice were inoculated with coxsackievirus B3. After sacrifice, the hearts were divided into 3 parts. Total RNA was extracted from one part of the heart. The other two parts were used for investigation of histopathology and of virus titer by plaque assay. The 32 P-labeled cDNA probe was derived from the 5′ end sequence of the coxsackievirus B3 genome. Northern blot autoradiograms of heart RNA were positive for viral RNA until day 7 and negative after day 10 and in control (uninfected) hearts. Positive autoradiograms always had the strongest signal at about 7.4 kilobase, corresponding to the size of the complete genome of the virus. The viral genomes were detected earlier than the appearance of the histopathologic changes in the murine heart. This type analysis of the viral genome in the murine myocardium may be useful in evaluating the effects of specific drugs on experimental viral myocarditis at the level of the viral genome.",
keywords = "Coxsackievirus B3, Murine myocarditis, Northern blotting, Viral genome, cDNA",
author = "Ikutaro Okada and Akira Matsumori and Chuichi Kawai and Junji Yodoi and Tracy, {Steven M}",
year = "1990",
month = "1",
day = "1",
doi = "10.1016/0022-2828(90)91039-A",
language = "English (US)",
volume = "22",
pages = "999--1008",
journal = "Journal of Molecular and Cellular Cardiology",
issn = "0022-2828",
publisher = "Academic Press Inc.",
number = "9",

}

TY - JOUR

T1 - The viral genome in experimental murine coxsackievirus B3 myocarditis

T2 - A Northern blotting anaylsis

AU - Okada, Ikutaro

AU - Matsumori, Akira

AU - Kawai, Chuichi

AU - Yodoi, Junji

AU - Tracy, Steven M

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The presence of viral genomes in the murine heart in experimental coxsackievirus B3 myocarditis was investigated by Northern blotting analysis. Four-week-old C3H He mice were inoculated with coxsackievirus B3. After sacrifice, the hearts were divided into 3 parts. Total RNA was extracted from one part of the heart. The other two parts were used for investigation of histopathology and of virus titer by plaque assay. The 32 P-labeled cDNA probe was derived from the 5′ end sequence of the coxsackievirus B3 genome. Northern blot autoradiograms of heart RNA were positive for viral RNA until day 7 and negative after day 10 and in control (uninfected) hearts. Positive autoradiograms always had the strongest signal at about 7.4 kilobase, corresponding to the size of the complete genome of the virus. The viral genomes were detected earlier than the appearance of the histopathologic changes in the murine heart. This type analysis of the viral genome in the murine myocardium may be useful in evaluating the effects of specific drugs on experimental viral myocarditis at the level of the viral genome.

AB - The presence of viral genomes in the murine heart in experimental coxsackievirus B3 myocarditis was investigated by Northern blotting analysis. Four-week-old C3H He mice were inoculated with coxsackievirus B3. After sacrifice, the hearts were divided into 3 parts. Total RNA was extracted from one part of the heart. The other two parts were used for investigation of histopathology and of virus titer by plaque assay. The 32 P-labeled cDNA probe was derived from the 5′ end sequence of the coxsackievirus B3 genome. Northern blot autoradiograms of heart RNA were positive for viral RNA until day 7 and negative after day 10 and in control (uninfected) hearts. Positive autoradiograms always had the strongest signal at about 7.4 kilobase, corresponding to the size of the complete genome of the virus. The viral genomes were detected earlier than the appearance of the histopathologic changes in the murine heart. This type analysis of the viral genome in the murine myocardium may be useful in evaluating the effects of specific drugs on experimental viral myocarditis at the level of the viral genome.

KW - Coxsackievirus B3

KW - Murine myocarditis

KW - Northern blotting

KW - Viral genome

KW - cDNA

UR - http://www.scopus.com/inward/record.url?scp=0025038619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025038619&partnerID=8YFLogxK

U2 - 10.1016/0022-2828(90)91039-A

DO - 10.1016/0022-2828(90)91039-A

M3 - Article

VL - 22

SP - 999

EP - 1008

JO - Journal of Molecular and Cellular Cardiology

JF - Journal of Molecular and Cellular Cardiology

SN - 0022-2828

IS - 9

ER -