The viral envelope gene is involved in macrophage tropism of a human immunodeficiency virus type 1 strain isolated from brain tissue

Z. Q. Liu, Charles Wood, J. A. Levy, C. Cheng-Mayer

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Abstract

Human immunodeficiency virus type 1 (HIV-1) strains isolated from the central nervous system (CNS) may represent a subgroup that displays a host cell tropism different from those isolated from peripheral blood and lymph nodes. One CNS-derived isolate, HIV-1(SF128A), which can be propagated efficiently in primary macrophage culture but not in any T-cell lines, was molecularly cloned and characterized. Recombinant viruses between HIV-1(SF128A) and the peripheral blood isolate HIV-1(SF2) were generated in order to map the viral gene(s) responsible for the macrophage tropism. The env gene sequences of the two isolates are about 91.1% homologous, with variations scattered mainly in the hypervariable regions of gp120. Recombinant viruses that have acquired the HIV-1(SF128A) env gene display HIV-1(SF128A) tropism for macrophages. Furthermore, the gp120 variable domains, V1, V2, V4, and V5, the CD4-binding domain, and the gp41 fusion domain are not directly involved in determining macrophage tropism.

Original languageEnglish (US)
Pages (from-to)6148-6153
Number of pages6
JournalJournal of virology
Volume64
Issue number12
StatePublished - Jan 1 1990

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tropisms
Tropism
Viral Genes
Human immunodeficiency virus 1
HIV-1
macrophages
Macrophages
brain
Brain
env Genes
genes
central nervous system
Viral Tropism
Central Nervous System
Viruses
viruses
blood
lymph nodes
tissues
T-lymphocytes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

The viral envelope gene is involved in macrophage tropism of a human immunodeficiency virus type 1 strain isolated from brain tissue. / Liu, Z. Q.; Wood, Charles; Levy, J. A.; Cheng-Mayer, C.

In: Journal of virology, Vol. 64, No. 12, 01.01.1990, p. 6148-6153.

Research output: Contribution to journalArticle

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