The use of two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF-MS) for metabolomic analysis of polar metabolites.

Kimberly Ralston-Hooper, Amber Jannasch, Jiri Adamec, Maria Sepúlveda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Metabolites produced by an organism can be quite extensive, and one analytical technique alone is not capable of their comprehensive detection and identification. The majority of environmental metabolomic studies have implemented proton nuclear magnetic resonance ((1)H-NMR) spectroscopy with little attention given to mass spectrometry (MS) techniques. In this chapter, an analytical technique is outlined that incorporates two-dimensional gas chromatography-time-of-flight MS (GC×GC-TOF-MS) for the identification and quantification of polar metabolites.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume708
DOIs
StatePublished - Apr 12 2011

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Metabolomics
Gas Chromatography
Mass Spectrometry
Magnetic Resonance Spectroscopy
Protons

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

The use of two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF-MS) for metabolomic analysis of polar metabolites. / Ralston-Hooper, Kimberly; Jannasch, Amber; Adamec, Jiri; Sepúlveda, Maria.

In: Methods in molecular biology (Clifton, N.J.), Vol. 708, 12.04.2011, p. 205-211.

Research output: Contribution to journalArticle

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