The use of scanning electron microscope autoradiography to localize the blueberry shoestring virus in its aphid vector

Karen L. Klomparens, Maureen A. Petersen, Donald C. Ramsdell, William G Chaney

Research output: Contribution to journalArticle

Abstract

Scanning electron microscopy autoradiography (SEM‐AR) in conjunction with light microscope autoradiography (LM‐AR) was used to follow the movement of 125I‐labeled blueberry shoestring virus (BBSSV) through its aphid vector Illinoia pepperi with varying acquisition access periods (AAP). At 6 hr AAP, the virus had reached the stomach; at 12 hr AAP, it had reached the anterior of the intestines, and after 48 hr, AAP was present throughout the aphid. SEM‐AR using backscatter electron detection proved very useful because the sample bulk resulted in a shortened exposure time compared to LM sections, correlation of the autoradiograms could be made with fine structure, preparing large numbers of samples was easy, and examining whole longitudinal sections of aphids at low magnification with a clearly visible marker was possible. Limitations were mostly attributed to sample preparation and, in some cases, were easily remedied.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalJournal of Electron Microscopy Technique
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1986

Fingerprint

Blueberry Plants
autoradiography
Aphids
viruses
Autoradiography
Viruses
acquisition
Electron microscopes
electron microscopes
Electrons
Scanning
Electron Scanning Microscopy
scanning
Scanning electron microscopy
stomach
intestines
scanning electron microscopy
Intestines
Stomach
Microscopes

Keywords

  • Aphid vector
  • Scanning electron microscope autoradiography
  • Virus localization

ASJC Scopus subject areas

  • Anatomy

Cite this

The use of scanning electron microscope autoradiography to localize the blueberry shoestring virus in its aphid vector. / Klomparens, Karen L.; Petersen, Maureen A.; Ramsdell, Donald C.; Chaney, William G.

In: Journal of Electron Microscopy Technique, Vol. 4, No. 1, 01.01.1986, p. 47-54.

Research output: Contribution to journalArticle

Klomparens, Karen L. ; Petersen, Maureen A. ; Ramsdell, Donald C. ; Chaney, William G. / The use of scanning electron microscope autoradiography to localize the blueberry shoestring virus in its aphid vector. In: Journal of Electron Microscopy Technique. 1986 ; Vol. 4, No. 1. pp. 47-54.
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