The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5′-UTR and first 92 ORF nucleotides

B. Kebaara, T. Nazarenus, R. Taylor, A. Forch, A. L. Atkin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

mRNAs containing premature translation termination codons (nonsense mRNAs) are targeted for deadenylation-independent degradation in a mechanism that depends on Upf1p, Upf2p and Upf3p. This decay pathway is often called nonsense-mediated mRNA decay (NMD). Nonsense mRNAs are decapped by Dcp1p and then degraded 5′ to 3′ by Xrn1p. In the yeast Saccharomyces cerevisiae, a significant number of wild-type mRNAs accumulate in upf mutants. Wild-type PPR1 mRNA is one of these mRNAs. Here we show that PPRI mRNA degradation depends on the Upf proteins, Dcp1p, Xrn1p and Hrp1p. We have mapped an Upf1p-dependent destabilizing element to a region located within the 5′-UTR and the first 92 bases of the PPR1 ORF. This element targets PPR1 mRNA for Upf-dependent decay by a novel mechanism.

Original languageEnglish (US)
Pages (from-to)3157-3165
Number of pages9
JournalNucleic acids research
Volume31
Issue number12
DOIs
StatePublished - Jun 15 2003

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5' Untranslated Regions
Open Reading Frames
Nucleotides
Messenger RNA
Nonsense Mediated mRNA Decay
Nonsense Codon
RNA Stability
Saccharomyces cerevisiae
Yeasts
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5′-UTR and first 92 ORF nucleotides. / Kebaara, B.; Nazarenus, T.; Taylor, R.; Forch, A.; Atkin, A. L.

In: Nucleic acids research, Vol. 31, No. 12, 15.06.2003, p. 3157-3165.

Research output: Contribution to journalArticle

Kebaara, B. ; Nazarenus, T. ; Taylor, R. ; Forch, A. ; Atkin, A. L. / The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5′-UTR and first 92 ORF nucleotides. In: Nucleic acids research. 2003 ; Vol. 31, No. 12. pp. 3157-3165.
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