The U-Box/ARM E3 ligase PUB13 regulates cell death, defense, and flowering time in Arabidops

Wei Li, Il Pyung Ahn, Yuese Ning, Chan Ho Park, Lirong Zeng, Justin G.A. Whitehill, Haibin Lu, Qingzhen Zhao, Bo Ding, Qi Xie, Jian Min Zhou, Liangying Dai, Guo Liang Wang

Research output: Contribution to journalArticle

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Abstract

The components in plant signal transduction pathways are intertwined and affect each other to coordinate plant growth, development, and defenses to stresses. The role of ubiquitination in connecting these pathways, particularly plant innate immunity and flowering, is largely unknown. Here, we report the dual roles for the Arabidopsis (Arabidopsis thaliana) Plant U-box protein13 (PUB13) in defense and flowering time control. In vitro ubiquitination assays indicated that PUB13 is an active E3 ubiquitin ligase and that the intact U-box domain is required for the E3 ligase activity. Disruption of the PUB13 gene by T-DNA insertion results in spontaneous cell death, the accumulation of hydrogen peroxide and salicylic acid (SA), and elevated resistance to biotrophic pathogens but increased susceptibility to necrotrophic pathogens. The cell death, hydrogen peroxide accumulation, and resistance to necrotrophic pathogens in pub13 are enhanced when plants are pretreated with high humidity. Importantly, pub13 also shows early flowering under middle and long-day conditions, in which the expression of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 and FLOWERING LOCUS T is induced while FLOWERING LOCUS C expression is suppressed. Finally, we found that two components involved in the SA-mediated signaling pathway, SID2 and PAD4, are required for the defense and flowering-time phenotypes caused by the loss of function of PUB13. Taken together, our data demonstrate that PUB13 acts as an important node connecting SA-dependent defense signaling and flowering time regulation in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)239-250
Number of pages12
JournalPlant physiology
Volume159
Issue number1
DOIs
StatePublished - May 1 2012

Fingerprint

Ubiquitin-Protein Ligases
ligases
cell death
Cell Death
flowering
Salicylic Acid
Arabidopsis
Ubiquitination
salicylic acid
Hydrogen Peroxide
Plant Immunity
hydrogen peroxide
Plant Structures
pathogens
Plant Development
Humidity
Growth and Development
Innate Immunity
ubiquitin-protein ligase
Signal Transduction

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Li, W., Ahn, I. P., Ning, Y., Park, C. H., Zeng, L., Whitehill, J. G. A., ... Wang, G. L. (2012). The U-Box/ARM E3 ligase PUB13 regulates cell death, defense, and flowering time in Arabidops. Plant physiology, 159(1), 239-250. https://doi.org/10.1104/pp.111.192617

The U-Box/ARM E3 ligase PUB13 regulates cell death, defense, and flowering time in Arabidops. / Li, Wei; Ahn, Il Pyung; Ning, Yuese; Park, Chan Ho; Zeng, Lirong; Whitehill, Justin G.A.; Lu, Haibin; Zhao, Qingzhen; Ding, Bo; Xie, Qi; Zhou, Jian Min; Dai, Liangying; Wang, Guo Liang.

In: Plant physiology, Vol. 159, No. 1, 01.05.2012, p. 239-250.

Research output: Contribution to journalArticle

Li, W, Ahn, IP, Ning, Y, Park, CH, Zeng, L, Whitehill, JGA, Lu, H, Zhao, Q, Ding, B, Xie, Q, Zhou, JM, Dai, L & Wang, GL 2012, 'The U-Box/ARM E3 ligase PUB13 regulates cell death, defense, and flowering time in Arabidops', Plant physiology, vol. 159, no. 1, pp. 239-250. https://doi.org/10.1104/pp.111.192617
Li, Wei ; Ahn, Il Pyung ; Ning, Yuese ; Park, Chan Ho ; Zeng, Lirong ; Whitehill, Justin G.A. ; Lu, Haibin ; Zhao, Qingzhen ; Ding, Bo ; Xie, Qi ; Zhou, Jian Min ; Dai, Liangying ; Wang, Guo Liang. / The U-Box/ARM E3 ligase PUB13 regulates cell death, defense, and flowering time in Arabidops. In: Plant physiology. 2012 ; Vol. 159, No. 1. pp. 239-250.
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