The tomato ubiquitin-conjugating enzyme variant Suv, but not SlUev1C and SlUev1D regulates fen-mediated programmed cell death in Nicotiana benthamiana

Sadia Hamera, Ravi M. Mural, Yao Liu, Lirong Zeng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The unconventional, lysine-63-linked ubiquitination has been shown to play a central role in regulating human and animal innate and adaptive immunity. By contrast, the role and mechanism of K63-linked ubiquitination in plant biology remain largely unexplored. The tomato (Solanum lycopersicum) Fni3 ubiquitin-conjugating enzyme and its cofactor, Suv ubiquitin E2 variant (Uev) were shown recently to catalyze K63-linked ubiquitination and are essential for protein Fen and other resistance protein-mediated plant immunity. In this study we detected the subcellular localization of Fen, Fni3 and Suv and confirmed the interaction of Fni3 with Suv in tomato protoplasts. Additionally, we identified 2 tomato Uev1 homologs, SlUev1C and SlUev1D, respectively and showed they are not required for Fen-mediated programmed cell death in Nicotiana benthamiana, suggesting Uev homologs play differential role in the cell.

Original languageEnglish (US)
Article numbere973814
JournalPlant Signaling and Behavior
Volume9
Issue number10
DOIs
StatePublished - Dec 22 2014

Fingerprint

ubiquitin-protein ligase
Nicotiana benthamiana
fens
apoptosis
tomatoes
ubiquitin
plant biology
Solanum lycopersicum
protoplasts
lysine
proteins
immunity
ubiquitination
animals
cells

Keywords

  • Cell death
  • K63-linked
  • Plant immunity
  • Ubiquitination
  • Uev

ASJC Scopus subject areas

  • Plant Science

Cite this

The tomato ubiquitin-conjugating enzyme variant Suv, but not SlUev1C and SlUev1D regulates fen-mediated programmed cell death in Nicotiana benthamiana. / Hamera, Sadia; Mural, Ravi M.; Liu, Yao; Zeng, Lirong.

In: Plant Signaling and Behavior, Vol. 9, No. 10, e973814, 22.12.2014.

Research output: Contribution to journalArticle

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abstract = "The unconventional, lysine-63-linked ubiquitination has been shown to play a central role in regulating human and animal innate and adaptive immunity. By contrast, the role and mechanism of K63-linked ubiquitination in plant biology remain largely unexplored. The tomato (Solanum lycopersicum) Fni3 ubiquitin-conjugating enzyme and its cofactor, Suv ubiquitin E2 variant (Uev) were shown recently to catalyze K63-linked ubiquitination and are essential for protein Fen and other resistance protein-mediated plant immunity. In this study we detected the subcellular localization of Fen, Fni3 and Suv and confirmed the interaction of Fni3 with Suv in tomato protoplasts. Additionally, we identified 2 tomato Uev1 homologs, SlUev1C and SlUev1D, respectively and showed they are not required for Fen-mediated programmed cell death in Nicotiana benthamiana, suggesting Uev homologs play differential role in the cell.",
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AU - Zeng, Lirong

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