The taurine biosynthetic pathway of microalgae

Rahul Tevatia, James Allen, Deepak Rudrappa, Derrick White, Thomas E. Clemente, Heriberto Cerutti, Yaşar Demirel, Paul Blum

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Taurine (2-aminoethanesulfonic acid) is an amino acid-like compound widely distributed in animals and an essential nutrient in some species. Targeted metabolomics of marine and fresh water microalgae combined with medium supplementation identified biosynthetic pathway intermediates and necessary catalytic activities. Genomic analysis was then used to predict the first taurine biosynthetic pathway in these organisms. MRM-based electrospray ionization (ESI) LC-MS/MS analysis demonstrated that taurine is synthesized using a carbon backbone from l-serine combined with sulfur derived from sulfate. Metabolite analysis showed a non-uniform pattern in levels of pathway intermediates that were both species and supplement-dependent. While increased culture salinity raised taurine levels modestly in marine alga, taurine levels were strongly induced in a fresh water species implicating taurine as an organic osmolyte. Conservation of the synthetic pathway in algae and metazoans together with a pattern of intermittent distribution in other lineages suggests that it arose early in eukaryotic evolution. Elevated levels of cell-associated taurine in algae could offer a new and biorenewable source of this unusual bioactive compound.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalAlgal Research
Volume9
DOIs
StatePublished - May 1 2015

Fingerprint

taurine
microalgae
biochemical pathways
algae
metabolomics
catalytic activity
serine
ionization
sulfates
sulfur
metabolites
salinity
genomics
amino acids
carbon
acids
organisms
nutrients
animals

Keywords

  • Bioinformatics
  • Microalgae
  • Osmolytes
  • Targeted metabolomics
  • Taurine

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Tevatia, R., Allen, J., Rudrappa, D., White, D., Clemente, T. E., Cerutti, H., ... Blum, P. (2015). The taurine biosynthetic pathway of microalgae. Algal Research, 9, 21-26. https://doi.org/10.1016/j.algal.2015.02.012

The taurine biosynthetic pathway of microalgae. / Tevatia, Rahul; Allen, James; Rudrappa, Deepak; White, Derrick; Clemente, Thomas E.; Cerutti, Heriberto; Demirel, Yaşar; Blum, Paul.

In: Algal Research, Vol. 9, 01.05.2015, p. 21-26.

Research output: Contribution to journalArticle

Tevatia, R, Allen, J, Rudrappa, D, White, D, Clemente, TE, Cerutti, H, Demirel, Y & Blum, P 2015, 'The taurine biosynthetic pathway of microalgae', Algal Research, vol. 9, pp. 21-26. https://doi.org/10.1016/j.algal.2015.02.012
Tevatia R, Allen J, Rudrappa D, White D, Clemente TE, Cerutti H et al. The taurine biosynthetic pathway of microalgae. Algal Research. 2015 May 1;9:21-26. https://doi.org/10.1016/j.algal.2015.02.012
Tevatia, Rahul ; Allen, James ; Rudrappa, Deepak ; White, Derrick ; Clemente, Thomas E. ; Cerutti, Heriberto ; Demirel, Yaşar ; Blum, Paul. / The taurine biosynthetic pathway of microalgae. In: Algal Research. 2015 ; Vol. 9. pp. 21-26.
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