The structure and function of replication protein A in DNA replication

Aishwarya Prakash, Gloria E Borgstahl

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPA’s roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.

Original languageEnglish (US)
Pages (from-to)171-196
Number of pages26
JournalSub-cellular biochemistry
Volume62
DOIs
StatePublished - Jan 1 2012

Fingerprint

Replication Protein A
DNA Replication
Single-Stranded DNA
DNA
DNA-Binding Proteins
DNA Repair
Proteins
Recombinational DNA Repair
Metabolism
Archaea
Repair
Eukaryota
Bacteria

Keywords

  • DNA replication
  • G-quadruplex
  • OB-fold
  • Protein-protein interaction
  • Replication protein A
  • Single-stranded DNA binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology
  • Cancer Research

Cite this

The structure and function of replication protein A in DNA replication. / Prakash, Aishwarya; Borgstahl, Gloria E.

In: Sub-cellular biochemistry, Vol. 62, 01.01.2012, p. 171-196.

Research output: Contribution to journalArticle

@article{069825e69343475abe77e0cde86f91aa,
title = "The structure and function of replication protein A in DNA replication",
abstract = "In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPA’s roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.",
keywords = "DNA replication, G-quadruplex, OB-fold, Protein-protein interaction, Replication protein A, Single-stranded DNA binding protein",
author = "Aishwarya Prakash and Borgstahl, {Gloria E}",
year = "2012",
month = "1",
day = "1",
doi = "10.1007/978-94-007-4572-8_10",
language = "English (US)",
volume = "62",
pages = "171--196",
journal = "Sub-Cellular Biochemistry",
issn = "0306-0225",
publisher = "Plenum Publishers",

}

TY - JOUR

T1 - The structure and function of replication protein A in DNA replication

AU - Prakash, Aishwarya

AU - Borgstahl, Gloria E

PY - 2012/1/1

Y1 - 2012/1/1

N2 - In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPA’s roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.

AB - In all organisms from bacteria and archaea to eukarya, single-stranded DNA binding proteins play an essential role in most, if not all, nuclear metabolism involving single-stranded DNA (ssDNA). Replication protein A (RPA), the major eukaryotic ssDNA binding protein, has two important roles in DNA metabolism: (1) in binding ssDNA to protect it and to keep it unfolded, and (2) in coordinating the assembly and disassembly of numerous proteins and protein complexes during processes such as DNA replication. Since its discovery as a vital player in the process of replication, RPA’s roles in recombination and DNA repair quickly became evident. This chapter summarizes the current understanding of RPA’s roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled.

KW - DNA replication

KW - G-quadruplex

KW - OB-fold

KW - Protein-protein interaction

KW - Replication protein A

KW - Single-stranded DNA binding protein

UR - http://www.scopus.com/inward/record.url?scp=84894489800&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894489800&partnerID=8YFLogxK

U2 - 10.1007/978-94-007-4572-8_10

DO - 10.1007/978-94-007-4572-8_10

M3 - Article

VL - 62

SP - 171

EP - 196

JO - Sub-Cellular Biochemistry

JF - Sub-Cellular Biochemistry

SN - 0306-0225

ER -