The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus

Narayanasamy Nandhagopal, Alan A. Simpson, James R. Gurnon, Xiadong Yan, Timothy S. Baker, Michael V. Graves, James L. Van Etten, Michael G. Rossmann

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a glycoprotein coat consisting of pseudohexagonal arrays of trimeric capsomers. Each capsomer is composed of three molecules of the major capsid protein, Vp54, the 2.0-A resolution structure of which is reported here. Four N-linked and two O-linked glycosylation sites were identified. The N-linked sites are associated with nonstandard amino acid motifs as a result of glycosylation by virus-encoded enzymes. Each monomer of the trimeric structure consists of two eight-stranded, antiparallel β-barrel, "jelly-roll" domains related by a pseudo-sixfold rotation. The fold of the monomer and the pseudo-sixfold symmetry of the capsomer resembles that of the major coat proteins in the double-stranded DNA bacteriophage PRD1 and the double-stranded DNA human adenoviruses, as well as the viral proteins VP2-VP3 of picornaviruses. The structural similarities among these diverse groups of viruses, whose hosts include bacteria, unicellular eukaryotes, plants, and mammals, make it probable that their capsid proteins have evolved from a common ancestor that had already acquired a pseudo-sixfold organization. The trimeric capsid protein structure was used to produce a quasi-atomic model of the 1,900-A diameter PBCV-1 outer shell, based on fitting of the Vp54 crystal structure into a three-dimensional cryoelectron microscopy image reconstruction of the virus.

Original languageEnglish (US)
Pages (from-to)14758-14763
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number23
DOIs
StatePublished - Nov 12 2002

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DNA Viruses
Capsid Proteins
Viruses
Lipids
Paramecium
Chlorella
Glycosylation
Bacteriophage PRD1
Picornaviridae
Cryoelectron Microscopy
Human Adenoviruses
Amino Acid Motifs
Computer-Assisted Image Processing
DNA
Viral Proteins
Eukaryota
Mammals
Glycoproteins
Bacteria
Membranes

ASJC Scopus subject areas

  • General

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The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus. / Nandhagopal, Narayanasamy; Simpson, Alan A.; Gurnon, James R.; Yan, Xiadong; Baker, Timothy S.; Graves, Michael V.; Van Etten, James L.; Rossmann, Michael G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 23, 12.11.2002, p. 14758-14763.

Research output: Contribution to journalArticle

Nandhagopal, Narayanasamy ; Simpson, Alan A. ; Gurnon, James R. ; Yan, Xiadong ; Baker, Timothy S. ; Graves, Michael V. ; Van Etten, James L. ; Rossmann, Michael G. / The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 23. pp. 14758-14763.
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