The Staphylococcus aureus scdA gene: A novel locus that affects cell division and morphogenesis

Eric W. Brunskill, Boudewijn L.M. De Jonge, Kenneth W. Bayles

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

A new Staphylococcus aureus gene termed scdA was found upstream of the autolysis regulatory genes, lytS and lytR, and was shown to potentially encode a hydrophilic 25 kDa protein. Analysis of scdA transcription revealed that it is transcribed as a monocistronic message and is lytSR-independent. A role in cell wall metabolism was indicated by examination of the scdA mutant S. aureus KB323, which had a grossly aberrant cellular morphology and formed large cell clusters when grown in liquid culture medium. Furthermore, KB323 exhibited a reduced rate of autolysis and had increased peptidoglycan cross-linking compared to the parental strain, NCTC 8325-4. These data suggest that scdA plays an important role in staphylococcal cell division.

Original languageEnglish (US)
Pages (from-to)2877-2882
Number of pages6
JournalMicrobiology
Volume143
Issue number9
DOIs
StatePublished - Sep 1997

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Keywords

  • Peptidoglycan cross-linking
  • Septum formation
  • Staphylococcus aureus
  • scdA

ASJC Scopus subject areas

  • Microbiology

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