The small glycine-rich RNA binding protein AtGRP7 promotes floral transition in Arabidopsis thaliana

Corinna Streitner, Selahattin Danisman, Franziska Wehrle, Jan C. Schöning, James R. Alfano, Dorothee Staiger

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The RNA binding protein AtGRP7 is part of a circadian slave oscillator in Arabidopsis thaliana that negatively autoregulates its own mRNA, and affects the levels of other transcripts. Here, we identify a novel role for AtGRP7 as a flowering-time gene. An atgrp7-1 T-DNA mutant flowers later than wild-type plants under both long and short days, and independent RNA interference lines with reduced levels of AtGRP7, and the closely related AtGRP8 protein, are also late flowering, particularly in short photoperiods. Consistent with the retention of a photoperiodic response, the transcript encoding the key photoperiodic regulator CONSTANS oscillates with a similar pattern in atgrp7-1 and wild-type plants. In both the RNAi lines and in the atgrp7-1 mutant transcript levels for the floral repressor FLC are elevated. Conversely, in transgenic plants ectopically overexpressing AtGRP7, the transition to flowering is accelerated mainly in short days, with a concomitant reduction in FLC abundance. The late-flowering phenotype of the RNAi lines is suppressed by introducing the flc-3 loss-of-function mutation, suggesting that AtGRP7 promotes floral transition, at least partly by downregulating FLC. Furthermore, vernalization overrides the late-flowering phenotype. Retention of both the photoperiodic response and vernalization response are features of autonomous pathway mutants, suggesting that AtGRP7 is a novel member of the autonomous pathway.

Original languageEnglish (US)
Pages (from-to)239-250
Number of pages12
JournalPlant Journal
Volume56
Issue number2
DOIs
StatePublished - Oct 1 2008

Fingerprint

RNA-binding proteins
RNA-Binding Proteins
RNA Interference
Arabidopsis
Glycine
Arabidopsis thaliana
flowering
vernalization
Phenotype
Genetically Modified Plants
mutants
Photoperiod
Down-Regulation
phenotype
Messenger RNA
Mutation
RNA interference
transgenic plants
photoperiod
Genes

Keywords

  • Arabidopsis
  • Autonomous pathway
  • Circadian clock
  • Flowering time
  • Post-transcriptional regulation
  • RNA binding protein

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

The small glycine-rich RNA binding protein AtGRP7 promotes floral transition in Arabidopsis thaliana. / Streitner, Corinna; Danisman, Selahattin; Wehrle, Franziska; Schöning, Jan C.; Alfano, James R.; Staiger, Dorothee.

In: Plant Journal, Vol. 56, No. 2, 01.10.2008, p. 239-250.

Research output: Contribution to journalArticle

Streitner, Corinna ; Danisman, Selahattin ; Wehrle, Franziska ; Schöning, Jan C. ; Alfano, James R. ; Staiger, Dorothee. / The small glycine-rich RNA binding protein AtGRP7 promotes floral transition in Arabidopsis thaliana. In: Plant Journal. 2008 ; Vol. 56, No. 2. pp. 239-250.
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