The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv

Anna Moroni, Carlo Viscomi, Vanessa Sangiorgio, Cinzia Pagliuca, Tobias Meckel, Ferenc Horvath, Sabrina Gazzarrini, Paola Valbuzzi, James L Van Etten, Dario DiFrancesco, Gerhard Thiel

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalFEBS Letters
Volume530
Issue number1-3
DOIs
StatePublished - Oct 23 2002

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Chlorella
Viruses
HEK293 Cells
Amino Acids
Membrane structures
Proteins
Membranes
Xenopus
Oocytes

Keywords

  • Chlorella virus PBCV-1
  • HEK 293 cell
  • K channel
  • Kcv

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Moroni, A., Viscomi, C., Sangiorgio, V., Pagliuca, C., Meckel, T., Horvath, F., ... Thiel, G. (2002). The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv. FEBS Letters, 530(1-3), 65-69. https://doi.org/10.1016/S0014-5793(02)03397-5

The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv. / Moroni, Anna; Viscomi, Carlo; Sangiorgio, Vanessa; Pagliuca, Cinzia; Meckel, Tobias; Horvath, Ferenc; Gazzarrini, Sabrina; Valbuzzi, Paola; Van Etten, James L; DiFrancesco, Dario; Thiel, Gerhard.

In: FEBS Letters, Vol. 530, No. 1-3, 23.10.2002, p. 65-69.

Research output: Contribution to journalArticle

Moroni, A, Viscomi, C, Sangiorgio, V, Pagliuca, C, Meckel, T, Horvath, F, Gazzarrini, S, Valbuzzi, P, Van Etten, JL, DiFrancesco, D & Thiel, G 2002, 'The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv', FEBS Letters, vol. 530, no. 1-3, pp. 65-69. https://doi.org/10.1016/S0014-5793(02)03397-5
Moroni, Anna ; Viscomi, Carlo ; Sangiorgio, Vanessa ; Pagliuca, Cinzia ; Meckel, Tobias ; Horvath, Ferenc ; Gazzarrini, Sabrina ; Valbuzzi, Paola ; Van Etten, James L ; DiFrancesco, Dario ; Thiel, Gerhard. / The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv. In: FEBS Letters. 2002 ; Vol. 530, No. 1-3. pp. 65-69.
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