The sequence of Giardia small subunit rRNA shows that voles and muskrats are parasitized by a unique species Giardia microti

Harry Van Keulen, Dennis E. Feely, P. Timothy Macechko, Edward L. Jarroll, Stanley L. Erlandsen

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The small subunit ribosomal RNA (eukaryotic 16S rRNA) gene from Giardia trophozoites, isolated from 8 different prairie voles and 8 different muskrats, was amplified by the polymerase chain reaction. The 16S rDNA was sequenced in its entirety for 2 prairie vole and 2 muskrat Giardia. In addition, the 5' 500 nucleotides of the 16S rDNA from Giardia isolates from each of 6 voles and 6 muskrats were amplified and sequenced. The results show that Giardia from voles and muskrats are very similar to each other but differ substantially from Giardia isolated from humans. We believe that the Giardia isolate from voles and muskrats constitutes a distinct species, which will be referred to as Giardia microti. These results suggest that both voles and muskrats are parasitized by the same species of Giardia, that this species is different from the Giardia that parasitizes humans, and that voles and muskrats do not contribute to the zoonotic character of human giardiasis.

Original languageEnglish (US)
Pages (from-to)294-300
Number of pages7
JournalJournal of Parasitology
Volume84
Issue number2
DOIs
StatePublished - Apr 1 1998

Fingerprint

Ondatra zibethicus
Giardia
Arvicolinae
ribosomal RNA
prairie
Microtus ochrogaster
polymerase chain reaction
gene
giardiasis
trophozoites
Giardia microti
voles
Ribosomal DNA
Eukaryotic Small Ribosome Subunits
nucleotides
Giardiasis
Trophozoites
Zoonoses

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

The sequence of Giardia small subunit rRNA shows that voles and muskrats are parasitized by a unique species Giardia microti. / Van Keulen, Harry; Feely, Dennis E.; Macechko, P. Timothy; Jarroll, Edward L.; Erlandsen, Stanley L.

In: Journal of Parasitology, Vol. 84, No. 2, 01.04.1998, p. 294-300.

Research output: Contribution to journalArticle

Van Keulen, Harry ; Feely, Dennis E. ; Macechko, P. Timothy ; Jarroll, Edward L. ; Erlandsen, Stanley L. / The sequence of Giardia small subunit rRNA shows that voles and muskrats are parasitized by a unique species Giardia microti. In: Journal of Parasitology. 1998 ; Vol. 84, No. 2. pp. 294-300.
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