The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain)

Janice C. Pedersen, Donald L. Reynolds, A. Ali

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 10 0.25 mean tissue culture infective dose (TCID 50 ) and 10 -0.44 TCID 50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.

Original languageEnglish (US)
Pages (from-to)681-685
Number of pages5
JournalAvian diseases
Volume44
Issue number3
DOIs
StatePublished - Jan 1 2000

Fingerprint

Metapneumovirus
Avian metapneumovirus
Carbon Monoxide
transcription (genetics)
Reverse Transcription
polymerase chain reaction
Sensitivity and Specificity
Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
assays
Pneumovirus
tissue culture
nucleotide sequences
dosage
Genes

Keywords

  • Avian pneumovirus
  • Reverse transcription-polymerase chain reaction
  • Swollen head syndrome
  • Turkey rhinotracheitis

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

The sensitivity and specificity of a severse transcription-polymerase chain reaction assay for the Avian Pneumovirus (Colorado strain). / Pedersen, Janice C.; Reynolds, Donald L.; Ali, A.

In: Avian diseases, Vol. 44, No. 3, 01.01.2000, p. 681-685.

Research output: Contribution to journalArticle

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