The role of Vasoactive Intestinal Peptide (VIP) in megakaryocyte proliferation

Chaneun Nam, Adam J. Case, Bruce S. Hostager, M. Sue O'Dorisio

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Megakaryocytopoiesis is a multistage process that involves differentiation of hematopoietic stem cells through the myeloid lineage, ultimately producing megakaryocytes and platelets. Vasoactive intestinal peptide (VIP) stimulates adenylate cyclase and induces differentiation in multiple cell types; VIP is expressed in hematopoietic stem cells and in megakaryocytes, but its function in these cells has not yet been delineated. The present study was designed to investigate whether the type 1 VIP receptor, VPAC1, mediates VIP effects on megakaryocytopoiesis. The human megakaryoblastic leukemia cell line (CMK) was transfected with VPAC1 and the transgene expression was confirmed by qualitative polymerase chain reaction and immunohistochemistry. The rate of proliferation and the patterns of differentiation were then compared for CMK and CMK/VPAC1 through multiple growth cycles. Upregulation of VPAC1 expression resulted in a decreased proliferation rate (p∈=∈0.0003) and enhanced differentiation with CMK/VPAC1 cells having twice the cell surface area of control CMK cells (p∈=∈0.001), thus increasing potential for proplatelet formation. These results suggest that VIP acts in an autocrine fashion via VPAC1 to inhibit megakaryocyte proliferation and induce proplatelet formation.

Original languageEnglish (US)
Pages (from-to)160-167
Number of pages8
JournalJournal of Molecular Neuroscience
Volume37
Issue number2
DOIs
StatePublished - Feb 1 2009

Fingerprint

Megakaryocytes
Vasoactive Intestinal Peptide
Thrombopoiesis
Hematopoietic Stem Cells
Receptors, Vasoactive Intestinal Polypeptide, Type I
Transgenes
Adenylyl Cyclases
Leukemia
Up-Regulation
Blood Platelets
Immunohistochemistry
Cell Line
Polymerase Chain Reaction
Growth

Keywords

  • CMK
  • Megakaryocyte
  • Platelet
  • Vasoactive intestinal peptide (VIP)
  • Vasoactive intestinal peptide receptor type 1 (VPAC1)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

The role of Vasoactive Intestinal Peptide (VIP) in megakaryocyte proliferation. / Nam, Chaneun; Case, Adam J.; Hostager, Bruce S.; O'Dorisio, M. Sue.

In: Journal of Molecular Neuroscience, Vol. 37, No. 2, 01.02.2009, p. 160-167.

Research output: Contribution to journalArticle

Nam, Chaneun ; Case, Adam J. ; Hostager, Bruce S. ; O'Dorisio, M. Sue. / The role of Vasoactive Intestinal Peptide (VIP) in megakaryocyte proliferation. In: Journal of Molecular Neuroscience. 2009 ; Vol. 37, No. 2. pp. 160-167.
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