The role of SATB1 in breast cancer pathogenesis

Elizabeth Iorns, H. James Hnatyszyn, Pearl Seo, Jennifer L Clarke, Toby Ward, Marc Lippman

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Background SATB1 has been previously proposed as a key protein that controls the development and progression of breast cancer. We explored the potential of the SATB1 protein as a therapeutic target and prognostic marker for human breast cancer.MethodsWe used aggressive (MDA-MB-231 and BT549) and nonaggressive (SKBR3 and MCF7) breast cancer cell lines to investigate the potential of SATB1 as a therapeutic target. SATB1 mRNA expression was silenced in aggressive cells by use of short hairpin RNAs against SATB1. SATB1 was overexpressed in nonaggressive cells by use of SATB1 expression vectors. We assessed the effect of modifying SATB1 expression on the transformed phenotype by examining anchorage-independent cell proliferation, acinar morphology on matrigel, and migration by wound healing in cultured cells. We examined tumor formation and metastasis, respectively, by use of orthotopic mammary fat pad and tail vein xenograft mouse models (mice were used in groups of six, and in total, 96 mice were used). SATB1 mRNA expression was compared with outcome for patients with primary breast cancer from six previous microarray studies that included a total of 1170 patients. All statistical tests were two-sided.ResultsThe transformed phenotype was not suppressed by SATB1 silencing in aggressive cells and was not enhanced by ectopic expression of SATB1 in nonaggressive cells. Modifying SATB1 expression did not alter anchorage-independent cell proliferation, invasive acinar morphology, or cell migration in cultured cells and did not affect tumor formation or metastasis in xenograft mouse models. In addition, SATB1 expression was not associated with decreased overall survival of patients with primary breast cancer in six previous independent microarray studies (overall odds ratio = 0.80, 95% confidence interval = 0.62 to 1.03, P =. 10).ConclusionIn contrast to previous studies, we found that SATB1 expression did not promote breast cancer progression and was not associated with breast cancer outcome.

Original languageEnglish (US)
Pages (from-to)1284-1296
Number of pages13
JournalJournal of the National Cancer Institute
Volume102
Issue number16
DOIs
StatePublished - Aug 18 2010

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Breast Neoplasms
Heterografts
Cultured Cells
Cell Proliferation
Neoplasm Metastasis
Phenotype
Messenger RNA
Wound Healing
Small Interfering RNA
Cell Movement
Tail
Adipose Tissue
Veins
Neoplasms
Proteins
Breast
Odds Ratio
Confidence Intervals
Cell Line
Survival

ASJC Scopus subject areas

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

Iorns, E., Hnatyszyn, H. J., Seo, P., Clarke, J. L., Ward, T., & Lippman, M. (2010). The role of SATB1 in breast cancer pathogenesis. Journal of the National Cancer Institute, 102(16), 1284-1296. https://doi.org/10.1093/jnci/djq243

The role of SATB1 in breast cancer pathogenesis. / Iorns, Elizabeth; Hnatyszyn, H. James; Seo, Pearl; Clarke, Jennifer L; Ward, Toby; Lippman, Marc.

In: Journal of the National Cancer Institute, Vol. 102, No. 16, 18.08.2010, p. 1284-1296.

Research output: Contribution to journalArticle

Iorns, E, Hnatyszyn, HJ, Seo, P, Clarke, JL, Ward, T & Lippman, M 2010, 'The role of SATB1 in breast cancer pathogenesis', Journal of the National Cancer Institute, vol. 102, no. 16, pp. 1284-1296. https://doi.org/10.1093/jnci/djq243
Iorns, Elizabeth ; Hnatyszyn, H. James ; Seo, Pearl ; Clarke, Jennifer L ; Ward, Toby ; Lippman, Marc. / The role of SATB1 in breast cancer pathogenesis. In: Journal of the National Cancer Institute. 2010 ; Vol. 102, No. 16. pp. 1284-1296.
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abstract = "Background SATB1 has been previously proposed as a key protein that controls the development and progression of breast cancer. We explored the potential of the SATB1 protein as a therapeutic target and prognostic marker for human breast cancer.MethodsWe used aggressive (MDA-MB-231 and BT549) and nonaggressive (SKBR3 and MCF7) breast cancer cell lines to investigate the potential of SATB1 as a therapeutic target. SATB1 mRNA expression was silenced in aggressive cells by use of short hairpin RNAs against SATB1. SATB1 was overexpressed in nonaggressive cells by use of SATB1 expression vectors. We assessed the effect of modifying SATB1 expression on the transformed phenotype by examining anchorage-independent cell proliferation, acinar morphology on matrigel, and migration by wound healing in cultured cells. We examined tumor formation and metastasis, respectively, by use of orthotopic mammary fat pad and tail vein xenograft mouse models (mice were used in groups of six, and in total, 96 mice were used). SATB1 mRNA expression was compared with outcome for patients with primary breast cancer from six previous microarray studies that included a total of 1170 patients. All statistical tests were two-sided.ResultsThe transformed phenotype was not suppressed by SATB1 silencing in aggressive cells and was not enhanced by ectopic expression of SATB1 in nonaggressive cells. Modifying SATB1 expression did not alter anchorage-independent cell proliferation, invasive acinar morphology, or cell migration in cultured cells and did not affect tumor formation or metastasis in xenograft mouse models. In addition, SATB1 expression was not associated with decreased overall survival of patients with primary breast cancer in six previous independent microarray studies (overall odds ratio = 0.80, 95{\%} confidence interval = 0.62 to 1.03, P =. 10).ConclusionIn contrast to previous studies, we found that SATB1 expression did not promote breast cancer progression and was not associated with breast cancer outcome.",
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