The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfotobus solfataricus

Viet Hoang, Elisabetta Bini, Vidula Dixit, Melissa Drozda, Paul Blum

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The archaeon Sulfolobus solfataricus uses a catabolite repression-like system to control production of several glycoside hydrolases. To better understand this regulatory system, studies of the regulation of expression of the β-glycosidase gene (lacS) were conducted. Expression of lacS varies in response to medium composition and to mutations at an unlinked gene called car. Despite gene overlap, expression of the lacS promoter proximal gene, SSO3017, exhibited coregulation but not cotranscription with lacS. Measurements of mRNA half-life excluded differential stability as a factor in lacS regulation. Chromosomal repositioning by homologous recombination of a lacS deletion series clarified critical cis-acting sequences required for lacS regulation. lacS repositioned at amyA exhibited increased lacS expression and compromised the response to medium composition independently of lacS 5′ flanking sequence composition. In contrast, regulation of lacS by the car mutation was dependent on sequences upstream of the archaeal TATA box. Expression of a promoter fusion between lacS and the car-independent malA promoter integrated either at amyA or at the natural lacS locus was insensitive to the allelic state of car. In contrast, the promoter fusion retained a response to medium composition only at the lacS locus. These results indicate that car acts at the lacS promoter and that the response to medium composition involves locus-specific sequences exclusive of those present 5′ to lacS or within the lacS transcription unit.

Original languageEnglish (US)
Pages (from-to)1563-1572
Number of pages10
JournalGenetics
Volume167
Issue number4
DOIs
StatePublished - Aug 1 2004

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Catabolite Repression
Glycoside Hydrolases
Archaea
Sulfolobus solfataricus
TATA Box
Mutation
5' Flanking Region
Homologous Recombination
Gene Expression Regulation
Genes
Half-Life
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfotobus solfataricus. / Hoang, Viet; Bini, Elisabetta; Dixit, Vidula; Drozda, Melissa; Blum, Paul.

In: Genetics, Vol. 167, No. 4, 01.08.2004, p. 1563-1572.

Research output: Contribution to journalArticle

Hoang, Viet ; Bini, Elisabetta ; Dixit, Vidula ; Drozda, Melissa ; Blum, Paul. / The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfotobus solfataricus. In: Genetics. 2004 ; Vol. 167, No. 4. pp. 1563-1572.
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