The role for NES1 serine protease as a novel tumor suppressor

Jaya Goyal, Karen M. Smith, Janet M. Cowan, David E. Wazer, Sam W. Lee, Vimla Band

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Previously (Liu et al., Cancer Res., 56: 3371-3379, 1996), we isolated a novel serine protease-like gene - Normal Epithelial Cell Specific-1 (NES1) - that is expressed in normal mammary epithelial cells but is down-regulated in most breast cancer cell lines. Here, we demonstrate that stable expression of NES1 in the NES1-negative MDA-MB-231 breast cancer cell line suppressed the oncogenicity as revealed by inhibition of the anchorage-independent growth and tumor formation in nude mice. Fluorescence in situ hybridization localized the NES1 gene to chromosome 19q13.3, a region that contains genes for related proteases (including the prostate-specific antigen) and is rearranged in human cancers. Similar to breast cancer cell lines, prostate cancer cell lines also lacked NES1 mRNA and protein expression. Together, these results strongly suggest a tumor-suppressor role for NES1 in breast and prostate cancer.

Original languageEnglish (US)
Pages (from-to)4782-4786
Number of pages5
JournalCancer Research
Volume58
Issue number21
StatePublished - Nov 1 1998

Fingerprint

Serine Proteases
Epithelial Cells
Neoplasms
Breast Neoplasms
Cell Line
Prostatic Neoplasms
Genes
Prostate-Specific Antigen
Fluorescence In Situ Hybridization
Nude Mice
Breast
Peptide Hydrolases
Chromosomes
Messenger RNA
Growth
Proteins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Goyal, J., Smith, K. M., Cowan, J. M., Wazer, D. E., Lee, S. W., & Band, V. (1998). The role for NES1 serine protease as a novel tumor suppressor. Cancer Research, 58(21), 4782-4786.

The role for NES1 serine protease as a novel tumor suppressor. / Goyal, Jaya; Smith, Karen M.; Cowan, Janet M.; Wazer, David E.; Lee, Sam W.; Band, Vimla.

In: Cancer Research, Vol. 58, No. 21, 01.11.1998, p. 4782-4786.

Research output: Contribution to journalArticle

Goyal, J, Smith, KM, Cowan, JM, Wazer, DE, Lee, SW & Band, V 1998, 'The role for NES1 serine protease as a novel tumor suppressor', Cancer Research, vol. 58, no. 21, pp. 4782-4786.
Goyal J, Smith KM, Cowan JM, Wazer DE, Lee SW, Band V. The role for NES1 serine protease as a novel tumor suppressor. Cancer Research. 1998 Nov 1;58(21):4782-4786.
Goyal, Jaya ; Smith, Karen M. ; Cowan, Janet M. ; Wazer, David E. ; Lee, Sam W. ; Band, Vimla. / The role for NES1 serine protease as a novel tumor suppressor. In: Cancer Research. 1998 ; Vol. 58, No. 21. pp. 4782-4786.
@article{4283fb7b8c5c4e178d84373c7c7b03a3,
title = "The role for NES1 serine protease as a novel tumor suppressor",
abstract = "Previously (Liu et al., Cancer Res., 56: 3371-3379, 1996), we isolated a novel serine protease-like gene - Normal Epithelial Cell Specific-1 (NES1) - that is expressed in normal mammary epithelial cells but is down-regulated in most breast cancer cell lines. Here, we demonstrate that stable expression of NES1 in the NES1-negative MDA-MB-231 breast cancer cell line suppressed the oncogenicity as revealed by inhibition of the anchorage-independent growth and tumor formation in nude mice. Fluorescence in situ hybridization localized the NES1 gene to chromosome 19q13.3, a region that contains genes for related proteases (including the prostate-specific antigen) and is rearranged in human cancers. Similar to breast cancer cell lines, prostate cancer cell lines also lacked NES1 mRNA and protein expression. Together, these results strongly suggest a tumor-suppressor role for NES1 in breast and prostate cancer.",
author = "Jaya Goyal and Smith, {Karen M.} and Cowan, {Janet M.} and Wazer, {David E.} and Lee, {Sam W.} and Vimla Band",
year = "1998",
month = "11",
day = "1",
language = "English (US)",
volume = "58",
pages = "4782--4786",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "21",

}

TY - JOUR

T1 - The role for NES1 serine protease as a novel tumor suppressor

AU - Goyal, Jaya

AU - Smith, Karen M.

AU - Cowan, Janet M.

AU - Wazer, David E.

AU - Lee, Sam W.

AU - Band, Vimla

PY - 1998/11/1

Y1 - 1998/11/1

N2 - Previously (Liu et al., Cancer Res., 56: 3371-3379, 1996), we isolated a novel serine protease-like gene - Normal Epithelial Cell Specific-1 (NES1) - that is expressed in normal mammary epithelial cells but is down-regulated in most breast cancer cell lines. Here, we demonstrate that stable expression of NES1 in the NES1-negative MDA-MB-231 breast cancer cell line suppressed the oncogenicity as revealed by inhibition of the anchorage-independent growth and tumor formation in nude mice. Fluorescence in situ hybridization localized the NES1 gene to chromosome 19q13.3, a region that contains genes for related proteases (including the prostate-specific antigen) and is rearranged in human cancers. Similar to breast cancer cell lines, prostate cancer cell lines also lacked NES1 mRNA and protein expression. Together, these results strongly suggest a tumor-suppressor role for NES1 in breast and prostate cancer.

AB - Previously (Liu et al., Cancer Res., 56: 3371-3379, 1996), we isolated a novel serine protease-like gene - Normal Epithelial Cell Specific-1 (NES1) - that is expressed in normal mammary epithelial cells but is down-regulated in most breast cancer cell lines. Here, we demonstrate that stable expression of NES1 in the NES1-negative MDA-MB-231 breast cancer cell line suppressed the oncogenicity as revealed by inhibition of the anchorage-independent growth and tumor formation in nude mice. Fluorescence in situ hybridization localized the NES1 gene to chromosome 19q13.3, a region that contains genes for related proteases (including the prostate-specific antigen) and is rearranged in human cancers. Similar to breast cancer cell lines, prostate cancer cell lines also lacked NES1 mRNA and protein expression. Together, these results strongly suggest a tumor-suppressor role for NES1 in breast and prostate cancer.

UR - http://www.scopus.com/inward/record.url?scp=0032212444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032212444&partnerID=8YFLogxK

M3 - Article

VL - 58

SP - 4782

EP - 4786

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 21

ER -