The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons

Z. H. He, H. T. Dong, J. X. Dong, D. B. Li, P. C. Ronald

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A rice transcript, Rim2, was identified that accumulated in both incompatible and compatible interactions between rice and Magnaporthe grisea. The Rim2 transcript also accumulated in response to treatment with a cell wall elicitor derived from M. grisea. A 3.3-kb RIM2 cDNA clone was isolated and is predicted to encode a protein of 653 amino acids, which shares 32-55% identity with TNP2-like proteins encoded by CACTA transposons of other plants. A 1.05-kb segment of the Rim2 sequence shows 82% nucleotide sequence identity with sequences flanking, the A1 and C members of the rice Xa21 disease resistance gene family. The 5'-upstream region of Rim2 was cloned and the transcriptional start sites were identified. The 5' and 3' noncoding termini of Rim2 are AT-rich. A cis-element showing similarity to a sequence that mediates defense-associated transcriptional activation of the tobacco retrotransposon Tnt1, and four motifs that fit the consensus sequence of the elicitor-responsive elements in the promoters of the parsley PR-1 genes were found in the 5'-upstream region. Four imperfect tandem repeats were identified in the 3' noncoding terminus. Southern analysis with genomic DNA from different rice species indicated that Rim2 is present in 3-4 copies per genome. These results suggest that Rim2 may be one component of a large CACTA-like element, whose transcript accumulates in response to attack by pathogens.

Original languageEnglish (US)
Pages (from-to)2-10
Number of pages9
JournalMolecular and General Genetics
Volume264
Issue number1-2
DOIs
StatePublished - Jan 1 2000

Fingerprint

Magnaporthe
Proteins
Petroselinum
Retroelements
Tandem Repeat Sequences
Disease Resistance
Consensus Sequence
Cell Wall
Transcriptional Activation
Genes
Tobacco
Complementary DNA
Clone Cells
Genome
Amino Acids
Oryza
DNA

Keywords

  • Induction
  • Magnaporthe grisea
  • Rice
  • Rim2
  • TNP2-like protein

ASJC Scopus subject areas

  • Genetics

Cite this

The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons. / He, Z. H.; Dong, H. T.; Dong, J. X.; Li, D. B.; Ronald, P. C.

In: Molecular and General Genetics, Vol. 264, No. 1-2, 01.01.2000, p. 2-10.

Research output: Contribution to journalArticle

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abstract = "A rice transcript, Rim2, was identified that accumulated in both incompatible and compatible interactions between rice and Magnaporthe grisea. The Rim2 transcript also accumulated in response to treatment with a cell wall elicitor derived from M. grisea. A 3.3-kb RIM2 cDNA clone was isolated and is predicted to encode a protein of 653 amino acids, which shares 32-55{\%} identity with TNP2-like proteins encoded by CACTA transposons of other plants. A 1.05-kb segment of the Rim2 sequence shows 82{\%} nucleotide sequence identity with sequences flanking, the A1 and C members of the rice Xa21 disease resistance gene family. The 5'-upstream region of Rim2 was cloned and the transcriptional start sites were identified. The 5' and 3' noncoding termini of Rim2 are AT-rich. A cis-element showing similarity to a sequence that mediates defense-associated transcriptional activation of the tobacco retrotransposon Tnt1, and four motifs that fit the consensus sequence of the elicitor-responsive elements in the promoters of the parsley PR-1 genes were found in the 5'-upstream region. Four imperfect tandem repeats were identified in the 3' noncoding terminus. Southern analysis with genomic DNA from different rice species indicated that Rim2 is present in 3-4 copies per genome. These results suggest that Rim2 may be one component of a large CACTA-like element, whose transcript accumulates in response to attack by pathogens.",
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