Zakonomernosti vosstanovleniia fenotipa dikogo tipa u transformantov drozhzhei Pichia guilliermondii.

Translated title of the contribution: The reversion of Pichia guilliermondii transformants to the wild-type phenotype

I. V. Piniaga, T. M. Prokopiv, A. V. Petrishin, Oleh Khalimonchuk, O. V. Protchenko, D. V. Fedorovich, I. R. Boretskii

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB7 of this enzyme. Experiments showed that the presence of an ARS element and the promoter region of the gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.

Original languageRussian
Pages (from-to)368-372
Number of pages5
JournalMikrobiologiia
Volume71
Issue number3
StatePublished - Jan 1 2002

Fingerprint

Pichia
GTP Cyclohydrolase
Phenotype
Genetic Promoter Regions
Genetic Recombination
Genes
Plasmids
Yeasts
Genome
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Piniaga, I. V., Prokopiv, T. M., Petrishin, A. V., Khalimonchuk, O., Protchenko, O. V., Fedorovich, D. V., & Boretskii, I. R. (2002). Zakonomernosti vosstanovleniia fenotipa dikogo tipa u transformantov drozhzhei Pichia guilliermondii. Mikrobiologiia, 71(3), 368-372.

Zakonomernosti vosstanovleniia fenotipa dikogo tipa u transformantov drozhzhei Pichia guilliermondii. / Piniaga, I. V.; Prokopiv, T. M.; Petrishin, A. V.; Khalimonchuk, Oleh; Protchenko, O. V.; Fedorovich, D. V.; Boretskii, I. R.

In: Mikrobiologiia, Vol. 71, No. 3, 01.01.2002, p. 368-372.

Research output: Contribution to journalArticle

Piniaga, IV, Prokopiv, TM, Petrishin, AV, Khalimonchuk, O, Protchenko, OV, Fedorovich, DV & Boretskii, IR 2002, 'Zakonomernosti vosstanovleniia fenotipa dikogo tipa u transformantov drozhzhei Pichia guilliermondii.', Mikrobiologiia, vol. 71, no. 3, pp. 368-372.
Piniaga IV, Prokopiv TM, Petrishin AV, Khalimonchuk O, Protchenko OV, Fedorovich DV et al. Zakonomernosti vosstanovleniia fenotipa dikogo tipa u transformantov drozhzhei Pichia guilliermondii. Mikrobiologiia. 2002 Jan 1;71(3):368-372.
Piniaga, I. V. ; Prokopiv, T. M. ; Petrishin, A. V. ; Khalimonchuk, Oleh ; Protchenko, O. V. ; Fedorovich, D. V. ; Boretskii, I. R. / Zakonomernosti vosstanovleniia fenotipa dikogo tipa u transformantov drozhzhei Pichia guilliermondii. In: Mikrobiologiia. 2002 ; Vol. 71, No. 3. pp. 368-372.
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