The respiratory tract microbial biogeography in alcohol use disorder

Derrick R. Samuelson, Ellen L. Burnham, Vincent J. Maffei, R. William Vandivier, Eugene E. Blanchard, Judd E. Shellito, Meng Luo, Christopher M. Taylor, David A. Welsh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Individuals with alcohol use disorders (AUDs) are at an increased risk of pneumonia and acute respiratory distress syndrome. Data of the lung microbiome in the setting of AUDs are lacking. The objective of this study was to determine the microbial biogeography of the upper and lower respiratory tract in individuals with AUDs compared with non-AUD subjects. Gargle, protected bronchial brush, and bronchoalveolar lavage specimens were collected during research bronchoscopies. Bacterial 16S gene sequencing and phylogenetic analysis was performed, and the alterations to the respiratory tract microbiota and changes in microbial biogeography were determined. The microbial structure of the upper and lower respiratory tract was significantly altered in subjects with AUDs compared with controls. Subjects with AUD have greater microbial diversity [P < 0.0001, effect size = 16 ± 1.7 observed taxa] and changes in microbial species relative abundances. Furthermore, microbial communities in the upper and lower respiratory tract displayed greater similarity in subjects with AUDs. Alcohol use is associated with an altered composition of the respiratory tract microbiota. Subjects with AUDs demonstrate convergence of the microbial phylogeny and taxonomic communities between distinct biogeographical sites within the respiratory tract. These results support a mechanistic pathway potentially explaining the increased incidence of pneumonia and lung diseases in patients with AUDs.

Original languageEnglish (US)
Pages (from-to)L107-L117
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume314
Issue number1
DOIs
StatePublished - Jan 2018

Fingerprint

Respiratory System
Alcohols
Microbiota
Bronchoalveolar Lavage
Pneumonia
Bacterial Genes
Adult Respiratory Distress Syndrome
Bronchoscopy
Phylogeny
Lung Diseases
Lung
Incidence
Research

Keywords

  • Alcohol
  • Biogeography
  • Lung
  • Microbiome
  • Respiratory

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Samuelson, D. R., Burnham, E. L., Maffei, V. J., Vandivier, R. W., Blanchard, E. E., Shellito, J. E., ... Welsh, D. A. (2018). The respiratory tract microbial biogeography in alcohol use disorder. American Journal of Physiology - Lung Cellular and Molecular Physiology, 314(1), L107-L117. https://doi.org/10.1152/ajplung.00277.2017

The respiratory tract microbial biogeography in alcohol use disorder. / Samuelson, Derrick R.; Burnham, Ellen L.; Maffei, Vincent J.; Vandivier, R. William; Blanchard, Eugene E.; Shellito, Judd E.; Luo, Meng; Taylor, Christopher M.; Welsh, David A.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 314, No. 1, 01.2018, p. L107-L117.

Research output: Contribution to journalArticle

Samuelson, DR, Burnham, EL, Maffei, VJ, Vandivier, RW, Blanchard, EE, Shellito, JE, Luo, M, Taylor, CM & Welsh, DA 2018, 'The respiratory tract microbial biogeography in alcohol use disorder', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 314, no. 1, pp. L107-L117. https://doi.org/10.1152/ajplung.00277.2017
Samuelson, Derrick R. ; Burnham, Ellen L. ; Maffei, Vincent J. ; Vandivier, R. William ; Blanchard, Eugene E. ; Shellito, Judd E. ; Luo, Meng ; Taylor, Christopher M. ; Welsh, David A. / The respiratory tract microbial biogeography in alcohol use disorder. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2018 ; Vol. 314, No. 1. pp. L107-L117.
@article{e81e34e76597419bae4223ae6be4395e,
title = "The respiratory tract microbial biogeography in alcohol use disorder",
abstract = "Individuals with alcohol use disorders (AUDs) are at an increased risk of pneumonia and acute respiratory distress syndrome. Data of the lung microbiome in the setting of AUDs are lacking. The objective of this study was to determine the microbial biogeography of the upper and lower respiratory tract in individuals with AUDs compared with non-AUD subjects. Gargle, protected bronchial brush, and bronchoalveolar lavage specimens were collected during research bronchoscopies. Bacterial 16S gene sequencing and phylogenetic analysis was performed, and the alterations to the respiratory tract microbiota and changes in microbial biogeography were determined. The microbial structure of the upper and lower respiratory tract was significantly altered in subjects with AUDs compared with controls. Subjects with AUD have greater microbial diversity [P < 0.0001, effect size = 16 ± 1.7 observed taxa] and changes in microbial species relative abundances. Furthermore, microbial communities in the upper and lower respiratory tract displayed greater similarity in subjects with AUDs. Alcohol use is associated with an altered composition of the respiratory tract microbiota. Subjects with AUDs demonstrate convergence of the microbial phylogeny and taxonomic communities between distinct biogeographical sites within the respiratory tract. These results support a mechanistic pathway potentially explaining the increased incidence of pneumonia and lung diseases in patients with AUDs.",
keywords = "Alcohol, Biogeography, Lung, Microbiome, Respiratory",
author = "Samuelson, {Derrick R.} and Burnham, {Ellen L.} and Maffei, {Vincent J.} and Vandivier, {R. William} and Blanchard, {Eugene E.} and Shellito, {Judd E.} and Meng Luo and Taylor, {Christopher M.} and Welsh, {David A.}",
year = "2018",
month = "1",
doi = "10.1152/ajplung.00277.2017",
language = "English (US)",
volume = "314",
pages = "L107--L117",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - The respiratory tract microbial biogeography in alcohol use disorder

AU - Samuelson, Derrick R.

AU - Burnham, Ellen L.

AU - Maffei, Vincent J.

AU - Vandivier, R. William

AU - Blanchard, Eugene E.

AU - Shellito, Judd E.

AU - Luo, Meng

AU - Taylor, Christopher M.

AU - Welsh, David A.

PY - 2018/1

Y1 - 2018/1

N2 - Individuals with alcohol use disorders (AUDs) are at an increased risk of pneumonia and acute respiratory distress syndrome. Data of the lung microbiome in the setting of AUDs are lacking. The objective of this study was to determine the microbial biogeography of the upper and lower respiratory tract in individuals with AUDs compared with non-AUD subjects. Gargle, protected bronchial brush, and bronchoalveolar lavage specimens were collected during research bronchoscopies. Bacterial 16S gene sequencing and phylogenetic analysis was performed, and the alterations to the respiratory tract microbiota and changes in microbial biogeography were determined. The microbial structure of the upper and lower respiratory tract was significantly altered in subjects with AUDs compared with controls. Subjects with AUD have greater microbial diversity [P < 0.0001, effect size = 16 ± 1.7 observed taxa] and changes in microbial species relative abundances. Furthermore, microbial communities in the upper and lower respiratory tract displayed greater similarity in subjects with AUDs. Alcohol use is associated with an altered composition of the respiratory tract microbiota. Subjects with AUDs demonstrate convergence of the microbial phylogeny and taxonomic communities between distinct biogeographical sites within the respiratory tract. These results support a mechanistic pathway potentially explaining the increased incidence of pneumonia and lung diseases in patients with AUDs.

AB - Individuals with alcohol use disorders (AUDs) are at an increased risk of pneumonia and acute respiratory distress syndrome. Data of the lung microbiome in the setting of AUDs are lacking. The objective of this study was to determine the microbial biogeography of the upper and lower respiratory tract in individuals with AUDs compared with non-AUD subjects. Gargle, protected bronchial brush, and bronchoalveolar lavage specimens were collected during research bronchoscopies. Bacterial 16S gene sequencing and phylogenetic analysis was performed, and the alterations to the respiratory tract microbiota and changes in microbial biogeography were determined. The microbial structure of the upper and lower respiratory tract was significantly altered in subjects with AUDs compared with controls. Subjects with AUD have greater microbial diversity [P < 0.0001, effect size = 16 ± 1.7 observed taxa] and changes in microbial species relative abundances. Furthermore, microbial communities in the upper and lower respiratory tract displayed greater similarity in subjects with AUDs. Alcohol use is associated with an altered composition of the respiratory tract microbiota. Subjects with AUDs demonstrate convergence of the microbial phylogeny and taxonomic communities between distinct biogeographical sites within the respiratory tract. These results support a mechanistic pathway potentially explaining the increased incidence of pneumonia and lung diseases in patients with AUDs.

KW - Alcohol

KW - Biogeography

KW - Lung

KW - Microbiome

KW - Respiratory

UR - http://www.scopus.com/inward/record.url?scp=85043458154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043458154&partnerID=8YFLogxK

U2 - 10.1152/ajplung.00277.2017

DO - 10.1152/ajplung.00277.2017

M3 - Article

C2 - 28860145

AN - SCOPUS:85043458154

VL - 314

SP - L107-L117

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 1

ER -