The respiratory tract microbial biogeography in alcohol use disorder

Derrick R. Samuelson, Ellen L. Burnham, Vincent J. Maffei, R. William Vandivier, Eugene E. Blanchard, Judd E. Shellito, Meng Luo, Christopher M. Taylor, David A. Welsh

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Individuals with alcohol use disorders (AUDs) are at an increased risk of pneumonia and acute respiratory distress syndrome. Data of the lung microbiome in the setting of AUDs are lacking. The objective of this study was to determine the microbial biogeography of the upper and lower respiratory tract in individuals with AUDs compared with non-AUD subjects. Gargle, protected bronchial brush, and bronchoalveolar lavage specimens were collected during research bronchoscopies. Bacterial 16S gene sequencing and phylogenetic analysis was performed, and the alterations to the respiratory tract microbiota and changes in microbial biogeography were determined. The microbial structure of the upper and lower respiratory tract was significantly altered in subjects with AUDs compared with controls. Subjects with AUD have greater microbial diversity [P < 0.0001, effect size = 16 ± 1.7 observed taxa] and changes in microbial species relative abundances. Furthermore, microbial communities in the upper and lower respiratory tract displayed greater similarity in subjects with AUDs. Alcohol use is associated with an altered composition of the respiratory tract microbiota. Subjects with AUDs demonstrate convergence of the microbial phylogeny and taxonomic communities between distinct biogeographical sites within the respiratory tract. These results support a mechanistic pathway potentially explaining the increased incidence of pneumonia and lung diseases in patients with AUDs.

Original languageEnglish (US)
Pages (from-to)L107-L117
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume314
Issue number1
DOIs
StatePublished - Jan 2018

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Keywords

  • Alcohol
  • Biogeography
  • Lung
  • Microbiome
  • Respiratory

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Samuelson, D. R., Burnham, E. L., Maffei, V. J., Vandivier, R. W., Blanchard, E. E., Shellito, J. E., Luo, M., Taylor, C. M., & Welsh, D. A. (2018). The respiratory tract microbial biogeography in alcohol use disorder. American Journal of Physiology - Lung Cellular and Molecular Physiology, 314(1), L107-L117. https://doi.org/10.1152/ajplung.00277.2017