The relative radiosensitivity of TK6 and WI-L2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status

W. Zhen, C. M. Denault, K. Loviscek, S. Walter, L. Geng, A. T.M. Vaughan

Research output: Contribution to journalArticle

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Abstract

The lymphoblastoid cell lines WI-L2-NS and TK6 were derived from a non-clonal pool of cells taken from a human spleen. Despite their common background they exhibit marked differences in radiosensitivities; D0 values of 93 and 67 cGy have been reported for WI-L2-NS and TK6 cells respectively. We show here that this differential radiosensitivity is due to a decreased ability of the WI-L2-NS cell line to undergo radiation-induced apoptosis. Further, the WI-L2-NS cell line overexpresses the p53 gene product as a result of a mutation in codon 237 of the p53 gene. These data indicate that WI-L2-NS cells through disruption of normal p53 function are unable to engage the radiation-induced apoptosis program and so are relatively radioresistant.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalMutation Research Letters
Volume346
Issue number2
DOIs
Publication statusPublished - Feb 1995

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Keywords

  • Apoptosis
  • Radiosensitivity
  • p53

ASJC Scopus subject areas

  • Medicine(all)

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