The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A

M. G. Brattain, C. M. Jones, J. M. Pittman, T. G. Pretlow

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Abstract

The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume65
Issue number1
DOIs
Publication statusPublished - Jul 8 1975

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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