The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A

Michael G Brattain, C. M. Jones, J. M. Pittman, T. G. Pretlow

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The partial purification of carcinoembryonic antigen from liver metastases by affinity chromatography with Concanavalin A is reported. Lyophilized perchloric acid extract of tumor tissue was resuspended and fractionated on Concanavalin A insolubilized by cross-linking with glutaraldehyde. Use of the insolubilized Con A in the batchwise method allowed for 65% recovery of the CEA with a 25-fold purification with regard to protein. The perchloric acid extracts contained 0.097 mg CEA per mg protein while the purest fraction from the insolubilized Con A contained 2.6 mg CEA per mg protein.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume65
Issue number1
DOIs
StatePublished - Jul 8 1975

Fingerprint

Carcinoembryonic Antigen
Glutaral
Concanavalin A
Purification
Affinity chromatography
Proteins
Tissue Extracts
Affinity Chromatography
Liver
Tumors
Tissue
Neoplasm Metastasis
Recovery
Neoplasms
Perchloric Acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A. / Brattain, Michael G; Jones, C. M.; Pittman, J. M.; Pretlow, T. G.

In: Biochemical and Biophysical Research Communications, Vol. 65, No. 1, 08.07.1975, p. 63-67.

Research output: Contribution to journalArticle

Brattain, Michael G ; Jones, C. M. ; Pittman, J. M. ; Pretlow, T. G. / The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A. In: Biochemical and Biophysical Research Communications. 1975 ; Vol. 65, No. 1. pp. 63-67.
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