The pgp1 mutant locus of Arabidopsis encodes a phosphatidylglycerolphosphate synthase with impaired activity

Changcheng Xu, Heiko Härtel, Hajime Wada, Miki Hagio, Bin Yu, Chris Eakin, Christoph Benning

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Phosphatidylglycerol is a ubiquitous phospholipid that is also present in the photosynthetic membranes of plants. Multiple independent lines of evidence suggest that this lipid plays a critical role for the proper function of photosynthetic membranes and cold acclimation. In eukaryotes, different subcellular compartments are competent for the biosynthesis of phosphatidylglycerol. Details on the plant-specific pathways in different organelles are scarce. Here, we describe a phosphatidylglycerol biosynthesis-deficient mutant of Arabidopsis, pgp1. The overall content of phosphatidylglycerol is reduced by 30%. This mutant carries a point mutation in the CDP-alcohol phosphotransferase motif of the phosphatidylglycerolphosphate synthase (EC 2.7.8.5) isoform encoded by a gene on chromosome 2. The mutant shows an 80% reduction in plastidic phosphatidylglycerolphosphate synthase activity consistent with the plastidic location of this particular isoform. Mutant plants are pale green, and their photosynthesis is impaired. This mutant provides a promising new tool to elucidate the biosynthesis and function of plastidic phosphatidylglycerol in seed plants.

Original languageEnglish (US)
Pages (from-to)594-604
Number of pages11
JournalPlant Physiology
Volume129
Issue number2
DOIs
StatePublished - Jan 1 2002

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Phosphatidylglycerols
phosphatidylglycerols
Arabidopsis
mutants
loci
biosynthesis
CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase
Protein Isoforms
Cytidine Diphosphate
Viridiplantae
Chromosomes, Human, Pair 2
Membranes
Acclimatization
Photosynthesis
point mutation
Spermatophytina
Eukaryota
Point Mutation
Organelles
organelles

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

The pgp1 mutant locus of Arabidopsis encodes a phosphatidylglycerolphosphate synthase with impaired activity. / Xu, Changcheng; Härtel, Heiko; Wada, Hajime; Hagio, Miki; Yu, Bin; Eakin, Chris; Benning, Christoph.

In: Plant Physiology, Vol. 129, No. 2, 01.01.2002, p. 594-604.

Research output: Contribution to journalArticle

Xu, Changcheng ; Härtel, Heiko ; Wada, Hajime ; Hagio, Miki ; Yu, Bin ; Eakin, Chris ; Benning, Christoph. / The pgp1 mutant locus of Arabidopsis encodes a phosphatidylglycerolphosphate synthase with impaired activity. In: Plant Physiology. 2002 ; Vol. 129, No. 2. pp. 594-604.
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