The PDZ binding motif of human papillomavirus type 16 E6 induces PTPN13 loss, which allows anchorage-independent growth and synergizes with ras for invasive growth

William Spanos, Andrew Hoover, George F. Harris, Shu Wu, Guinevere L. Strand, Mary E. Anderson, Aloysius J. Klingelhutz, Wiljan Hendriks, Aaron D. Bossler, John H. Lee

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Abstract

The human papillomavirus (HPV) oncogene E6 has been shown to perform multiple functions (p53 degradation, telomerase activation, etc.) that play a role in oncogenic transformation. Beyond known E6 functions, an undefined mechanism that allows cellular invasion requires the E6 PDZ binding motif (PDZBM). Here, we show that HPV type 16 (HPV16) E6 interacts with and induces loss of a protein tyrosine phosphatase (PTPN13) in a PDZBM-dependent manner. PTPN13 loss induced either by the presence of E6 or by a short hairpin RNA strategy allows for anchorage-independent growth (AIG) and synergy with a known oncogene, Rasv12, resulting in invasive growth in vivo. Restoring PTPN13 expression reverses AIG in cells lacking PTPN13. A genomic analysis of colorectal carcinoma has identified an association between PTPN13 loss-of-function mutations and aberrant Ras signaling. Our findings support this correlation and provide methods for further evaluation of the mechanisms by which PTPN13 loss/Ras expression leads to invasive growth, the results of which will be important for treatment of HPV-related and non-HPV cancer.

Original languageEnglish (US)
Pages (from-to)2493-2500
Number of pages8
JournalJournal of virology
Volume82
Issue number5
DOIs
StatePublished - Mar 1 2008

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Human papillomavirus 16
Papillomaviridae
oncogenes
Growth
Oncogenes
protein-tyrosine-phosphatase
telomerase
Protein Tyrosine Phosphatases
Telomerase
small interfering RNA
colorectal neoplasms
Small Interfering RNA
cell growth
Colorectal Neoplasms
genomics
Mutation
neoplasms
degradation
Neoplasms
methodology

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

The PDZ binding motif of human papillomavirus type 16 E6 induces PTPN13 loss, which allows anchorage-independent growth and synergizes with ras for invasive growth. / Spanos, William; Hoover, Andrew; Harris, George F.; Wu, Shu; Strand, Guinevere L.; Anderson, Mary E.; Klingelhutz, Aloysius J.; Hendriks, Wiljan; Bossler, Aaron D.; Lee, John H.

In: Journal of virology, Vol. 82, No. 5, 01.03.2008, p. 2493-2500.

Research output: Contribution to journalArticle

Spanos, W, Hoover, A, Harris, GF, Wu, S, Strand, GL, Anderson, ME, Klingelhutz, AJ, Hendriks, W, Bossler, AD & Lee, JH 2008, 'The PDZ binding motif of human papillomavirus type 16 E6 induces PTPN13 loss, which allows anchorage-independent growth and synergizes with ras for invasive growth', Journal of virology, vol. 82, no. 5, pp. 2493-2500. https://doi.org/10.1128/JVI.02188-07
Spanos, William ; Hoover, Andrew ; Harris, George F. ; Wu, Shu ; Strand, Guinevere L. ; Anderson, Mary E. ; Klingelhutz, Aloysius J. ; Hendriks, Wiljan ; Bossler, Aaron D. ; Lee, John H. / The PDZ binding motif of human papillomavirus type 16 E6 induces PTPN13 loss, which allows anchorage-independent growth and synergizes with ras for invasive growth. In: Journal of virology. 2008 ; Vol. 82, No. 5. pp. 2493-2500.
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