The pattern of gene expression in mouse Gr-1+ myeloid progenitor cells

Jianjun Chen, Donald A. Rowley, Terry Clark, Sanggyu Lee, Guolin Zhou, Christian Beck, Janet D. Rowley, San Ming Wang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To understand the pattern of gene expression in mouse myeloid progenitor cells, we carried out a genome-wide analysis of gene expression in mouse bone marrow Gr-1+ cells using SAGE and GLGI techniques. We identified 22,033 unique SAGE tags with quantitative information from 73,869 collected SAGE tags. Among these unique tags, 64% match known sequences, including many genes important for myeloid differentiation, and 36% have no matches to known sequences and are likely to represent novel genes. We compared the expression of mouse Gr-1+ and human CD15+ myeloid progenitor cells and showed that the pattern of gene expression of these two cell populations had some similarities. We also compared the expression of mouse Gr-1+ myeloid progenitor cells with that of mouse brain tissue and found a highly tissue-specific manner of gene expression in these two samples. Our data provide a basis for studying altered gene expression in myeloid disorders using mouse models.

Original languageEnglish (US)
Pages (from-to)149-162
Number of pages14
JournalGenomics
Volume77
Issue number3
DOIs
StatePublished - Aug 15 2001

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Myeloid Progenitor Cells
Gene Expression
Genes
Bone Marrow
Genome
Brain
Population

Keywords

  • GLGI technique
  • Gene expression
  • Gene profile
  • Genome
  • Novel genes
  • SAGE technique

ASJC Scopus subject areas

  • Genetics

Cite this

Chen, J., Rowley, D. A., Clark, T., Lee, S., Zhou, G., Beck, C., ... Wang, S. M. (2001). The pattern of gene expression in mouse Gr-1+ myeloid progenitor cells. Genomics, 77(3), 149-162. https://doi.org/10.1006/geno.2001.6633

The pattern of gene expression in mouse Gr-1+ myeloid progenitor cells. / Chen, Jianjun; Rowley, Donald A.; Clark, Terry; Lee, Sanggyu; Zhou, Guolin; Beck, Christian; Rowley, Janet D.; Wang, San Ming.

In: Genomics, Vol. 77, No. 3, 15.08.2001, p. 149-162.

Research output: Contribution to journalArticle

Chen, J, Rowley, DA, Clark, T, Lee, S, Zhou, G, Beck, C, Rowley, JD & Wang, SM 2001, 'The pattern of gene expression in mouse Gr-1+ myeloid progenitor cells', Genomics, vol. 77, no. 3, pp. 149-162. https://doi.org/10.1006/geno.2001.6633
Chen J, Rowley DA, Clark T, Lee S, Zhou G, Beck C et al. The pattern of gene expression in mouse Gr-1+ myeloid progenitor cells. Genomics. 2001 Aug 15;77(3):149-162. https://doi.org/10.1006/geno.2001.6633
Chen, Jianjun ; Rowley, Donald A. ; Clark, Terry ; Lee, Sanggyu ; Zhou, Guolin ; Beck, Christian ; Rowley, Janet D. ; Wang, San Ming. / The pattern of gene expression in mouse Gr-1+ myeloid progenitor cells. In: Genomics. 2001 ; Vol. 77, No. 3. pp. 149-162.
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