The pattern of gene expression in human CD15+ myeloid progenitor cells

Sanggyu Lee, Guolin Zhou, Terry Clark, Jianjun Chen, Janet D. Rowley, San Ming Wang

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We performed a genome-wide analysis of gene expression in primary human CD15+ myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high and intermediate levels, whereas 75% were present as single copies, (ii) 53% of the tags matched known expressed sequences, 34% of which were matched to more than one known expressed sequence, and (iii) 47% of the tags had no matches and represent potentially novel genes. The correct genes were confirmed by application of the generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) technique for high-copy tags with multiple matches. A set of genes known to be important in myeloid differentiation were expressed at various levels and used different spliced forms. This study provides a normal baseline for comparison of gene expression in myeloid diseases. The strategy of using SAGE and GLGI techniques in this study has broad applications to the genome-wide identification of expressed genes.

Original languageEnglish (US)
Pages (from-to)3340-3345
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number6
DOIs
StatePublished - Mar 13 2001

Fingerprint

Myeloid Progenitor Cells
Gene Expression
Genes
Genome
Complementary DNA

ASJC Scopus subject areas

  • General

Cite this

The pattern of gene expression in human CD15+ myeloid progenitor cells. / Lee, Sanggyu; Zhou, Guolin; Clark, Terry; Chen, Jianjun; Rowley, Janet D.; Wang, San Ming.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 6, 13.03.2001, p. 3340-3345.

Research output: Contribution to journalArticle

Lee, Sanggyu ; Zhou, Guolin ; Clark, Terry ; Chen, Jianjun ; Rowley, Janet D. ; Wang, San Ming. / The pattern of gene expression in human CD15+ myeloid progenitor cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 6. pp. 3340-3345.
@article{6414d7d811f041e5af3dbe17d54a5f01,
title = "The pattern of gene expression in human CD15+ myeloid progenitor cells",
abstract = "We performed a genome-wide analysis of gene expression in primary human CD15+ myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25{\%} were detected at high and intermediate levels, whereas 75{\%} were present as single copies, (ii) 53{\%} of the tags matched known expressed sequences, 34{\%} of which were matched to more than one known expressed sequence, and (iii) 47{\%} of the tags had no matches and represent potentially novel genes. The correct genes were confirmed by application of the generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) technique for high-copy tags with multiple matches. A set of genes known to be important in myeloid differentiation were expressed at various levels and used different spliced forms. This study provides a normal baseline for comparison of gene expression in myeloid diseases. The strategy of using SAGE and GLGI techniques in this study has broad applications to the genome-wide identification of expressed genes.",
author = "Sanggyu Lee and Guolin Zhou and Terry Clark and Jianjun Chen and Rowley, {Janet D.} and Wang, {San Ming}",
year = "2001",
month = "3",
day = "13",
doi = "10.1073/pnas.051013798",
language = "English (US)",
volume = "98",
pages = "3340--3345",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "6",

}

TY - JOUR

T1 - The pattern of gene expression in human CD15+ myeloid progenitor cells

AU - Lee, Sanggyu

AU - Zhou, Guolin

AU - Clark, Terry

AU - Chen, Jianjun

AU - Rowley, Janet D.

AU - Wang, San Ming

PY - 2001/3/13

Y1 - 2001/3/13

N2 - We performed a genome-wide analysis of gene expression in primary human CD15+ myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high and intermediate levels, whereas 75% were present as single copies, (ii) 53% of the tags matched known expressed sequences, 34% of which were matched to more than one known expressed sequence, and (iii) 47% of the tags had no matches and represent potentially novel genes. The correct genes were confirmed by application of the generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) technique for high-copy tags with multiple matches. A set of genes known to be important in myeloid differentiation were expressed at various levels and used different spliced forms. This study provides a normal baseline for comparison of gene expression in myeloid diseases. The strategy of using SAGE and GLGI techniques in this study has broad applications to the genome-wide identification of expressed genes.

AB - We performed a genome-wide analysis of gene expression in primary human CD15+ myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high and intermediate levels, whereas 75% were present as single copies, (ii) 53% of the tags matched known expressed sequences, 34% of which were matched to more than one known expressed sequence, and (iii) 47% of the tags had no matches and represent potentially novel genes. The correct genes were confirmed by application of the generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) technique for high-copy tags with multiple matches. A set of genes known to be important in myeloid differentiation were expressed at various levels and used different spliced forms. This study provides a normal baseline for comparison of gene expression in myeloid diseases. The strategy of using SAGE and GLGI techniques in this study has broad applications to the genome-wide identification of expressed genes.

UR - http://www.scopus.com/inward/record.url?scp=0035853090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035853090&partnerID=8YFLogxK

U2 - 10.1073/pnas.051013798

DO - 10.1073/pnas.051013798

M3 - Article

VL - 98

SP - 3340

EP - 3345

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 6

ER -