The octamer motif present in the rex-1 promoter binds oct-1 and oct-3 expressed by EC cells and ES cells

E. Rosfjord, A Angie Rizzino

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer family of transcription factors that stimulates expression from the Rex-1 promoter. In this report, we utilized gel mobility shift analysis to examine the binding of transcription factors to the Rex-1 octamer motif. F9 EC cells, D3 ES cells, and human NT2/D1 EC cells each form at least two prominent DNA/protein complexes with the octamer motif. Supershift analysis identifies Oct-1 and Oct-3 in these complexes. When F9 EC cells are induced to differentiate by treatment with RA for 48 h, there is a complete loss of the DNA/protein complex containing Oct-3. In contrast, the other DNA/protein complexes, including the DNA/protein complex containing Oct-1, can still be detected. These results provide support for a role of Oct-3 in the transcription of the Rex-1 gene.

Original languageEnglish (US)
Pages (from-to)1795-1802
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number3
DOIs
StatePublished - Sep 30 1994

Fingerprint

Embryonal Carcinoma Stem Cells
pX Genes
Embryonic Stem Cells
Stem cells
Genes
DNA
Transcription
Tretinoin
Octamer Transcription Factors
Proteins
Transcription Factors
5' Flanking Region
Zinc Fingers
Electrophoretic Mobility Shift Assay
Zinc
Gels
Cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The octamer motif present in the rex-1 promoter binds oct-1 and oct-3 expressed by EC cells and ES cells. / Rosfjord, E.; Rizzino, A Angie.

In: Biochemical and Biophysical Research Communications, Vol. 203, No. 3, 30.09.1994, p. 1795-1802.

Research output: Contribution to journalArticle

@article{86c85daf07e84ceb8b00e786f7a26324,
title = "The octamer motif present in the rex-1 promoter binds oct-1 and oct-3 expressed by EC cells and ES cells",
abstract = "Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer family of transcription factors that stimulates expression from the Rex-1 promoter. In this report, we utilized gel mobility shift analysis to examine the binding of transcription factors to the Rex-1 octamer motif. F9 EC cells, D3 ES cells, and human NT2/D1 EC cells each form at least two prominent DNA/protein complexes with the octamer motif. Supershift analysis identifies Oct-1 and Oct-3 in these complexes. When F9 EC cells are induced to differentiate by treatment with RA for 48 h, there is a complete loss of the DNA/protein complex containing Oct-3. In contrast, the other DNA/protein complexes, including the DNA/protein complex containing Oct-1, can still be detected. These results provide support for a role of Oct-3 in the transcription of the Rex-1 gene.",
author = "E. Rosfjord and Rizzino, {A Angie}",
year = "1994",
month = "9",
day = "30",
doi = "10.1006/bbrc.1994.2395",
language = "English (US)",
volume = "203",
pages = "1795--1802",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The octamer motif present in the rex-1 promoter binds oct-1 and oct-3 expressed by EC cells and ES cells

AU - Rosfjord, E.

AU - Rizzino, A Angie

PY - 1994/9/30

Y1 - 1994/9/30

N2 - Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer family of transcription factors that stimulates expression from the Rex-1 promoter. In this report, we utilized gel mobility shift analysis to examine the binding of transcription factors to the Rex-1 octamer motif. F9 EC cells, D3 ES cells, and human NT2/D1 EC cells each form at least two prominent DNA/protein complexes with the octamer motif. Supershift analysis identifies Oct-1 and Oct-3 in these complexes. When F9 EC cells are induced to differentiate by treatment with RA for 48 h, there is a complete loss of the DNA/protein complex containing Oct-3. In contrast, the other DNA/protein complexes, including the DNA/protein complex containing Oct-1, can still be detected. These results provide support for a role of Oct-3 in the transcription of the Rex-1 gene.

AB - Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer family of transcription factors that stimulates expression from the Rex-1 promoter. In this report, we utilized gel mobility shift analysis to examine the binding of transcription factors to the Rex-1 octamer motif. F9 EC cells, D3 ES cells, and human NT2/D1 EC cells each form at least two prominent DNA/protein complexes with the octamer motif. Supershift analysis identifies Oct-1 and Oct-3 in these complexes. When F9 EC cells are induced to differentiate by treatment with RA for 48 h, there is a complete loss of the DNA/protein complex containing Oct-3. In contrast, the other DNA/protein complexes, including the DNA/protein complex containing Oct-1, can still be detected. These results provide support for a role of Oct-3 in the transcription of the Rex-1 gene.

UR - http://www.scopus.com/inward/record.url?scp=0028067959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028067959&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1994.2395

DO - 10.1006/bbrc.1994.2395

M3 - Article

VL - 203

SP - 1795

EP - 1802

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -