The nonequilibrium character of DNA melting

Effects of the heating rate on the fine structure of melting curves

Sergei A. Kozyavkin, Yuri L Lyubchenko

Research output: Contribution to journalArticle

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Abstract

To investigate the effects of heating rate on the DNA melting profile and to test the predictions of the theory of slow relaxation processes in DNA melting (1) concerning these effects, we obtained differential melting curves for the Bsp I C1 fragment of T7 DNA (1461 bp) and the Sma I-Eco RI fragment of Col E1 DNA (1291 bp) at heating rates of 0.05 and 0.5 deg/min. At low ionic strength (0.02 M Na+ ) the heating rate has been shown to affect the position of the third peak in melting curve for C1 fragment. According to the melting maps (2), this peak corresponds to the unwinding of the section between the end of the molecule and he region already melted. At high ionic strength (0.2 M Na+ ), when the melting of this DNA is reversible (3), the position of the peaks does not depend on the heating rate. In the case of the Col E1 DNA fragment the heating rate affects, as might be expected from the melting maps (4), only the last peak, as the melting of the last section is always nonequilibrium. The results of the study are in good qualitative agreement and in satisfactory quantitative agreement with the theoretical predictions (1).

Original languageEnglish (US)
Pages (from-to)4339-4349
Number of pages11
JournalNucleic acids research
Volume12
Issue number10
DOIs
StatePublished - May 25 1984

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Nucleic Acid Denaturation
Fine Structure
Heating rate
Melting
Heating
Freezing
Non-equilibrium
DNA
Curve
Fragment
Osmolar Concentration
Deoxyribonuclease EcoRI
Ionic strength
Character
Fine structure
Prediction
Relaxation processes
Molecules

ASJC Scopus subject areas

  • Genetics

Cite this

The nonequilibrium character of DNA melting : Effects of the heating rate on the fine structure of melting curves. / Kozyavkin, Sergei A.; Lyubchenko, Yuri L.

In: Nucleic acids research, Vol. 12, No. 10, 25.05.1984, p. 4339-4349.

Research output: Contribution to journalArticle

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AB - To investigate the effects of heating rate on the DNA melting profile and to test the predictions of the theory of slow relaxation processes in DNA melting (1) concerning these effects, we obtained differential melting curves for the Bsp I C1 fragment of T7 DNA (1461 bp) and the Sma I-Eco RI fragment of Col E1 DNA (1291 bp) at heating rates of 0.05 and 0.5 deg/min. At low ionic strength (0.02 M Na+ ) the heating rate has been shown to affect the position of the third peak in melting curve for C1 fragment. According to the melting maps (2), this peak corresponds to the unwinding of the section between the end of the molecule and he region already melted. At high ionic strength (0.2 M Na+ ), when the melting of this DNA is reversible (3), the position of the peaks does not depend on the heating rate. In the case of the Col E1 DNA fragment the heating rate affects, as might be expected from the melting maps (4), only the last peak, as the melting of the last section is always nonequilibrium. The results of the study are in good qualitative agreement and in satisfactory quantitative agreement with the theoretical predictions (1).

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