The neuroprotection of KIBRA in promoting neuron survival and against amyloid β-induced apoptosis

Lin Song, Shi Tang, Lingling Dong, Xiaolei Han, Lin Cong, Jixin Dong, Xiaojuan Han, Qinghua Zhang, Yongxiang Wang, Yifeng Du

Research output: Contribution to journalArticle

Abstract

Background: Recent research has identified the nucleotide polymorphisms of KIdney and BRAin expressed protein (KIBRA) to be associated with cognitive performance, suggesting its vital role in Alzheimer’s disease (AD); however, the underlying molecular mechanism of KIBRA in AD remains obscure. Methods: The AD animal model (APP/PS1 transgenic mice) and KIBRA knockout (KIBRA KO) mice were used to investigate pathophysiological changes of KIBRA in vivo. Mouse hippocampal cell line (HT22) was used to explore its molecular mechanism through KIBRA CRISPR/Cas9-sgRNA system and KIBRA overexpression lentivirus in vitro. Results: Aged APP/PS1 mice displayed increased neuronal apoptosis in the hippocampus, as did KIBRA KO mice. KIBRA deficiency was closely related to neuronal loss in the brain. In addition, knockdown of KIBRA in neuronal cell lines suppressed its growth and elevated apoptosis-associated protein levels under the stress of Aβ1-42 oligomers. On the contrary, overexpression of KIBRA significantly promoted cell proliferation and reduced its apoptosis. Moreover, through screening several survival-related signaling pathways, we found that KIBRA inhibited apoptosis by activating the Akt pathway other than ERK or PKC pathways, which was further confirmed by Akt-specific inhibitor MK2206. Conclusion: Our data indicate that KIBRA may function as a neuroprotective gene in promoting neuron survival and inhibiting Aβ-induced neuronal apoptosis.

Original languageEnglish (US)
Article number137
JournalFrontiers in Cellular Neuroscience
Volume13
DOIs
StatePublished - Apr 12 2019

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Amyloid
Apoptosis
Neurons
Alzheimer Disease
Knockout Mice
Clustered Regularly Interspaced Short Palindromic Repeats
Cell Line
Lentivirus
MAP Kinase Signaling System
Transgenic Mice
Hippocampus
Proteins
Nucleotides
Animal Models
Cell Proliferation
Neuroprotection
Kidney
Brain
Growth
Research

Keywords

  • Alzheimer’s disease
  • Amyloid-β
  • Apoptosis
  • KIBRA
  • Neuroprotection

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

The neuroprotection of KIBRA in promoting neuron survival and against amyloid β-induced apoptosis. / Song, Lin; Tang, Shi; Dong, Lingling; Han, Xiaolei; Cong, Lin; Dong, Jixin; Han, Xiaojuan; Zhang, Qinghua; Wang, Yongxiang; Du, Yifeng.

In: Frontiers in Cellular Neuroscience, Vol. 13, 137, 12.04.2019.

Research output: Contribution to journalArticle

Song, Lin ; Tang, Shi ; Dong, Lingling ; Han, Xiaolei ; Cong, Lin ; Dong, Jixin ; Han, Xiaojuan ; Zhang, Qinghua ; Wang, Yongxiang ; Du, Yifeng. / The neuroprotection of KIBRA in promoting neuron survival and against amyloid β-induced apoptosis. In: Frontiers in Cellular Neuroscience. 2019 ; Vol. 13.
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abstract = "Background: Recent research has identified the nucleotide polymorphisms of KIdney and BRAin expressed protein (KIBRA) to be associated with cognitive performance, suggesting its vital role in Alzheimer’s disease (AD); however, the underlying molecular mechanism of KIBRA in AD remains obscure. Methods: The AD animal model (APP/PS1 transgenic mice) and KIBRA knockout (KIBRA KO) mice were used to investigate pathophysiological changes of KIBRA in vivo. Mouse hippocampal cell line (HT22) was used to explore its molecular mechanism through KIBRA CRISPR/Cas9-sgRNA system and KIBRA overexpression lentivirus in vitro. Results: Aged APP/PS1 mice displayed increased neuronal apoptosis in the hippocampus, as did KIBRA KO mice. KIBRA deficiency was closely related to neuronal loss in the brain. In addition, knockdown of KIBRA in neuronal cell lines suppressed its growth and elevated apoptosis-associated protein levels under the stress of Aβ1-42 oligomers. On the contrary, overexpression of KIBRA significantly promoted cell proliferation and reduced its apoptosis. Moreover, through screening several survival-related signaling pathways, we found that KIBRA inhibited apoptosis by activating the Akt pathway other than ERK or PKC pathways, which was further confirmed by Akt-specific inhibitor MK2206. Conclusion: Our data indicate that KIBRA may function as a neuroprotective gene in promoting neuron survival and inhibiting Aβ-induced neuronal apoptosis.",
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