The mitogen-activated protein kinase scaffold KSR1 is required for recruitment of extracellular signal-regulated kinase to the immunological synapse

Emanuele Giurisato, Joseph Lin, Angus Harding, Elisa Cerutti, Marina Cella, Robert E Lewis, Marco Colonna, Andrey S. Shaw

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

KSR1 is a mitogen-activated protein (MAP) kinase scaffold that enhances the activation of the MAP kinase extracellular signal-regulated kinase (ERK). The function of KSR1 in NK cell function is not known. Here we show that KSR1 is required for efficient NK-mediated cytolysis and polarization of cytolytic granules. Singlecell analysis showed that ERK is activated in an all-or-none fashion in both wild-type and KSR1-deficient cells. In the absence of KSR1, however, the efficiency of ERK activation is attenuated. Imaging studies showed that KSR1 is recruited to the immunological synapse during T-cell activation and that membrane recruitment of KSR1 is required for recruitment of active ERK to the synapse.

Original languageEnglish (US)
Pages (from-to)1554-1564
Number of pages11
JournalMolecular and cellular biology
Volume29
Issue number6
DOIs
StatePublished - Mar 1 2009

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Immunological Synapses
Extracellular Signal-Regulated MAP Kinases
Mitogen-Activated Protein Kinases
Natural Killer Cells
Synapses
Cell Membrane
T-Lymphocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The mitogen-activated protein kinase scaffold KSR1 is required for recruitment of extracellular signal-regulated kinase to the immunological synapse. / Giurisato, Emanuele; Lin, Joseph; Harding, Angus; Cerutti, Elisa; Cella, Marina; Lewis, Robert E; Colonna, Marco; Shaw, Andrey S.

In: Molecular and cellular biology, Vol. 29, No. 6, 01.03.2009, p. 1554-1564.

Research output: Contribution to journalArticle

Giurisato, Emanuele ; Lin, Joseph ; Harding, Angus ; Cerutti, Elisa ; Cella, Marina ; Lewis, Robert E ; Colonna, Marco ; Shaw, Andrey S. / The mitogen-activated protein kinase scaffold KSR1 is required for recruitment of extracellular signal-regulated kinase to the immunological synapse. In: Molecular and cellular biology. 2009 ; Vol. 29, No. 6. pp. 1554-1564.
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