The medium-/long-chain fatty acyl-CoA dehydrogenase (fadF) gene of Salmonella typhimurium is a phase 1 starvation-stress response (SSR) locus

Michael P. Spector, Concetta C. DiRusso, Mark J. Pallen, Francisco Garcia Del Portillo, Gordon Dougan, B. Brett Finlay

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Salmonella enterica serovar Typhimurium (S. typhimurium) is an enteric pathogen that causes significant morbidity in humans and other mammals. During their life cycle, salmonellae must survive frequent exposures to a variety of environmental stresses, e.g, carbon-source (C) starvation. The starvation-stress response (SSR) of S. typhimurium encompasses the genetic and physiological realignments that occur when an essential nutrient becomes limiting for bacterial growth. The function of the SSR is to produce a cell capable of surviving long-term starvation. This paper reports that three C-starvation-inducible lac fusions from an S. typhimurium C-starvation-inducible lac fusion library are all within a gene identified as fadF, which encodes an acyl-CoA dehydrogenase (ACDH) specific for medium-/long-chain fatty acids. This identification is supported by several findings: (a) significant homology at the amino acid sequence level with the ACDH enzymes from other bacteria and eukaryotes, (b) undetectable β-oxidation levels in fadF insertion mutants, (c) inability of fad insertion mutants to grow on oleate or decanoate as a sole C-source, and (d) inducibility of fadF::lac fusions by the long-chain fatty acid oleate. In addition, the results indicate that the C-starvation-induction of fadF is under negative control by the FadR global regulator and positive control by the cAMP:cAMP receptor protein complex and ppGpp. It is also shown that the fadF locus is important for C-starvation-survival in S. typhimurium. Furthermore, the results demonstrate that fadF is induced within cultured Madin-Darby canine kidney (MDCK) epithelial cells, suggesting that signals for its induction (C-starvation and/or long-chain fatty acids) may be present in the intracellular environment encountered by S. typhimurium. However, fadF insertion mutations did not have an overt effect on mouse virulence.

Original languageEnglish (US)
Pages (from-to)15-31
Number of pages17
JournalMicrobiology
Volume145
Issue number1
DOIs
StatePublished - Jan 1999

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Long-Chain Acyl-CoA Dehydrogenase
Acyl-CoA Dehydrogenase
Salmonella typhimurium
Starvation
Genes
Fatty Acids
Oleic Acid
Cyclic AMP Receptor Protein
Decanoates
Amino Acid Sequence Homology
Madin Darby Canine Kidney Cells
Salmonella enterica
Insertional Mutagenesis
Life Cycle Stages
Eukaryota
Salmonella
Libraries
Virulence
Mammals
Cultured Cells

Keywords

  • Fatty acid degradation
  • Starvation-stress response
  • cAMP-receptor protein
  • fadR
  • ppGpp

ASJC Scopus subject areas

  • Microbiology

Cite this

The medium-/long-chain fatty acyl-CoA dehydrogenase (fadF) gene of Salmonella typhimurium is a phase 1 starvation-stress response (SSR) locus. / Spector, Michael P.; DiRusso, Concetta C.; Pallen, Mark J.; Garcia Del Portillo, Francisco; Dougan, Gordon; Finlay, B. Brett.

In: Microbiology, Vol. 145, No. 1, 01.1999, p. 15-31.

Research output: Contribution to journalArticle

Spector, Michael P. ; DiRusso, Concetta C. ; Pallen, Mark J. ; Garcia Del Portillo, Francisco ; Dougan, Gordon ; Finlay, B. Brett. / The medium-/long-chain fatty acyl-CoA dehydrogenase (fadF) gene of Salmonella typhimurium is a phase 1 starvation-stress response (SSR) locus. In: Microbiology. 1999 ; Vol. 145, No. 1. pp. 15-31.
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AU - Dougan, Gordon

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