The mechanism of microsomal hydroxylation of 7-methylbenz[a]-anthracene and 7,12-dimethylbenz[a]anthracene by oxygen-18 studies

C. Grandjean, Ercole Cavalieri

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.

Original languageEnglish (US)
Pages (from-to)912-919
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume61
Issue number3
DOIs
StatePublished - Dec 11 1974

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Hydroxylation
Oxygen
High pressure liquid chromatography
Derivatives
Oxygenases
Liver Microsomes
Metabolites
Isotopes
Liver
Mass spectrometry
Rats
Mass Spectrometry
High Pressure Liquid Chromatography
7-methylbenzanthracene
anthracene

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.",
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T1 - The mechanism of microsomal hydroxylation of 7-methylbenz[a]-anthracene and 7,12-dimethylbenz[a]anthracene by oxygen-18 studies

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AU - Cavalieri, Ercole

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AB - The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.

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