The LysR-type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus

Sujata S. Chaudhari, Vinai Chittezham Thomas, Marat R. Sadykov, Jeffrey L. Bose, Daniel J. Ahn, Matthew C Zimmerman, Kenneth W Bayles

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Staphylococcus aureus LysR-type transcriptional regulator, CidR, activates the expression of two operons including cidABC and alsSD that display pro- and anti-death functions, respectively. Although several investigations have focused on the functions of different genes associated with these operons, the collective role of the CidR regulon in staphylococcal physiology is not clearly understood. Here we reveal that the primary role of this regulon is to limit acetate-dependent potentiation of cell death in staphylococcal populations. Although both CidB and CidC promote acetate generation and cell death, the CidR-dependent co-activation of CidA and AlsSD counters the effects of CidBC by redirecting intracellular carbon flux towards acetoin formation. From a mechanistic standpoint, we demonstrate that CidB is necessary for full activation of CidC, whereas CidA limits the abundance of CidC in the cell.

Original languageEnglish (US)
Pages (from-to)942-953
Number of pages12
JournalMolecular Microbiology
Volume101
Issue number6
DOIs
StatePublished - Sep 1 2016

Fingerprint

Regulon
Operon
Staphylococcus aureus
Acetates
Cell Death
Acetoin
Carbon Cycle
Population
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

The LysR-type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus. / Chaudhari, Sujata S.; Chittezham Thomas, Vinai; Sadykov, Marat R.; Bose, Jeffrey L.; Ahn, Daniel J.; Zimmerman, Matthew C; Bayles, Kenneth W.

In: Molecular Microbiology, Vol. 101, No. 6, 01.09.2016, p. 942-953.

Research output: Contribution to journalArticle

@article{a52ce457ed1043239d21139eb6548d33,
title = "The LysR-type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus",
abstract = "The Staphylococcus aureus LysR-type transcriptional regulator, CidR, activates the expression of two operons including cidABC and alsSD that display pro- and anti-death functions, respectively. Although several investigations have focused on the functions of different genes associated with these operons, the collective role of the CidR regulon in staphylococcal physiology is not clearly understood. Here we reveal that the primary role of this regulon is to limit acetate-dependent potentiation of cell death in staphylococcal populations. Although both CidB and CidC promote acetate generation and cell death, the CidR-dependent co-activation of CidA and AlsSD counters the effects of CidBC by redirecting intracellular carbon flux towards acetoin formation. From a mechanistic standpoint, we demonstrate that CidB is necessary for full activation of CidC, whereas CidA limits the abundance of CidC in the cell.",
author = "Chaudhari, {Sujata S.} and {Chittezham Thomas}, Vinai and Sadykov, {Marat R.} and Bose, {Jeffrey L.} and Ahn, {Daniel J.} and Zimmerman, {Matthew C} and Bayles, {Kenneth W}",
year = "2016",
month = "9",
day = "1",
doi = "10.1111/mmi.13433",
language = "English (US)",
volume = "101",
pages = "942--953",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - The LysR-type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus

AU - Chaudhari, Sujata S.

AU - Chittezham Thomas, Vinai

AU - Sadykov, Marat R.

AU - Bose, Jeffrey L.

AU - Ahn, Daniel J.

AU - Zimmerman, Matthew C

AU - Bayles, Kenneth W

PY - 2016/9/1

Y1 - 2016/9/1

N2 - The Staphylococcus aureus LysR-type transcriptional regulator, CidR, activates the expression of two operons including cidABC and alsSD that display pro- and anti-death functions, respectively. Although several investigations have focused on the functions of different genes associated with these operons, the collective role of the CidR regulon in staphylococcal physiology is not clearly understood. Here we reveal that the primary role of this regulon is to limit acetate-dependent potentiation of cell death in staphylococcal populations. Although both CidB and CidC promote acetate generation and cell death, the CidR-dependent co-activation of CidA and AlsSD counters the effects of CidBC by redirecting intracellular carbon flux towards acetoin formation. From a mechanistic standpoint, we demonstrate that CidB is necessary for full activation of CidC, whereas CidA limits the abundance of CidC in the cell.

AB - The Staphylococcus aureus LysR-type transcriptional regulator, CidR, activates the expression of two operons including cidABC and alsSD that display pro- and anti-death functions, respectively. Although several investigations have focused on the functions of different genes associated with these operons, the collective role of the CidR regulon in staphylococcal physiology is not clearly understood. Here we reveal that the primary role of this regulon is to limit acetate-dependent potentiation of cell death in staphylococcal populations. Although both CidB and CidC promote acetate generation and cell death, the CidR-dependent co-activation of CidA and AlsSD counters the effects of CidBC by redirecting intracellular carbon flux towards acetoin formation. From a mechanistic standpoint, we demonstrate that CidB is necessary for full activation of CidC, whereas CidA limits the abundance of CidC in the cell.

UR - http://www.scopus.com/inward/record.url?scp=84985930029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84985930029&partnerID=8YFLogxK

U2 - 10.1111/mmi.13433

DO - 10.1111/mmi.13433

M3 - Article

C2 - 27253847

AN - SCOPUS:84985930029

VL - 101

SP - 942

EP - 953

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 6

ER -