The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria

Aaron Atkinson, Pamela Smith, Jennifer L. Fox, Tie Zhong Cui, Oleh Khalimonchuk, Dennis R. Winge

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The assembly of the cytochrome bc 1 complex in Saccharomyces cerevisiae is shown to be conditionally dependent on a novel factor, Mzm1. Cells lacking Mzm1 exhibit a modest bc 1 defect at 30°C, but the defect is exacerbated at elevated temperatures. Formation of bc 1 is stalled in mzm1Δ cells at a late assembly intermediate lacking the Rieske iron-sulfur protein Rip1. Rip1 levels are markedly attenuated in mzm1Δ cells at elevated temperatures. Respiratory growth can be restored in the mutant cells by the overexpression of the Rip1 subunit. Elevated levels of Mzm1 enhance the stabilization of Rip1 through physical interaction, suggesting that Mzm1 may be an important Rip1 chaperone especially under heat stress. Mzm1 may function primarily to stabilize Rip1 prior to inner membrane (IM) insertion or alternatively to aid in the presentation of Rip1 to the inner membrane translocation complex for extrusion of the folded domain containing the iron-sulfur center.

Original languageEnglish (US)
Pages (from-to)3988-3996
Number of pages9
JournalMolecular and cellular biology
Volume31
Issue number19
DOIs
StatePublished - Oct 1 2011

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Fungal Proteins
Protein S
Mitochondria
Proteins
Temperature
Membranes
Cytochromes
Sulfur
Saccharomyces cerevisiae
Iron
Hot Temperature
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria. / Atkinson, Aaron; Smith, Pamela; Fox, Jennifer L.; Cui, Tie Zhong; Khalimonchuk, Oleh; Winge, Dennis R.

In: Molecular and cellular biology, Vol. 31, No. 19, 01.10.2011, p. 3988-3996.

Research output: Contribution to journalArticle

Atkinson, Aaron ; Smith, Pamela ; Fox, Jennifer L. ; Cui, Tie Zhong ; Khalimonchuk, Oleh ; Winge, Dennis R. / The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria. In: Molecular and cellular biology. 2011 ; Vol. 31, No. 19. pp. 3988-3996.
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