The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans

Thaila Fernanda Dos Reis, Benjamin M. Nitsche, Pollyne Borborema Almeida De Lima, Leandro José De Assis, Laura Mellado, Steven D Harris, Vera Meyer, Renato A.Corrêa Dos Santos, Diego M. Riaño-Pachón, Laure Nicolas Annick Ries, Gustavo H. Goldman

Research output: Contribution to journalArticle

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Abstract

One of the drawbacks during second-generation biofuel production from plant lignocellulosic biomass is the accumulation of glucose, the preferred carbon source of microorganisms, which causes the repression of hydrolytic enzyme secretion by industrially relevant filamentous fungi. Glucose sensing, subsequent transport and cellular signalling pathways have been barely elucidated in these organisms. This study therefore characterized the transcriptional response of the filamentous fungus Aspergillus nidulans to the presence of high and low glucose concentrations under continuous chemostat cultivation with the aim to identify novel factors involved in glucose sensing and signalling. Several transcription factor-and transporter-encoding genes were identified as being differentially regulated, including the previously characterized glucose and xylose transporter HxtB. HxtB was confirmed to be a low affinity glucose transporter, localizing to the plasma membrane under low-and high-glucose conditions. Furthermore, HxtB was shown to be involved in conidiation-related processes and may play a role in downstream glucose signalling. A gene predicted to encode the protein kinase PskA was also identified as being important for glucose metabolism. This study identified several proteins with predicted roles in glucose metabolic processes and provides a foundation for further investigation into the response of biotechnologically important filamentous fungi to glucose.

Original languageEnglish (US)
Article number45073
JournalScientific Reports
Volume7
DOIs
StatePublished - Mar 31 2017

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Aspergillus nidulans
Facilitative Glucose Transport Proteins
Glucose
Fungi
Enzyme Repression
Biofuels
Biomass
Protein Kinases
Genes
Transcription Factors
Carbon
Cell Membrane

ASJC Scopus subject areas

  • General

Cite this

Dos Reis, T. F., Nitsche, B. M., De Lima, P. B. A., De Assis, L. J., Mellado, L., Harris, S. D., ... Goldman, G. H. (2017). The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans. Scientific Reports, 7, [45073]. https://doi.org/10.1038/srep45073

The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans. / Dos Reis, Thaila Fernanda; Nitsche, Benjamin M.; De Lima, Pollyne Borborema Almeida; De Assis, Leandro José; Mellado, Laura; Harris, Steven D; Meyer, Vera; Dos Santos, Renato A.Corrêa; Riaño-Pachón, Diego M.; Ries, Laure Nicolas Annick; Goldman, Gustavo H.

In: Scientific Reports, Vol. 7, 45073, 31.03.2017.

Research output: Contribution to journalArticle

Dos Reis, TF, Nitsche, BM, De Lima, PBA, De Assis, LJ, Mellado, L, Harris, SD, Meyer, V, Dos Santos, RAC, Riaño-Pachón, DM, Ries, LNA & Goldman, GH 2017, 'The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans', Scientific Reports, vol. 7, 45073. https://doi.org/10.1038/srep45073
Dos Reis, Thaila Fernanda ; Nitsche, Benjamin M. ; De Lima, Pollyne Borborema Almeida ; De Assis, Leandro José ; Mellado, Laura ; Harris, Steven D ; Meyer, Vera ; Dos Santos, Renato A.Corrêa ; Riaño-Pachón, Diego M. ; Ries, Laure Nicolas Annick ; Goldman, Gustavo H. / The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans. In: Scientific Reports. 2017 ; Vol. 7.
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