The locus encompassing the latency-associated transcript of herpes simplex virus type 1 interferes with and delays interferon expression in productively infected neuroblastoma cells and trigeminal ganglia of acutely infected mice

Weiping Peng, Gail Henderson, Melissa Inman, Lbachir BenMohamed, Guey Chuen Perng, Steven L. Wechsler, Clinton Jones

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) is the only abundant viral transcript expressed in latently infected neurons. LAT inhibits apoptosis, suggesting that it regulates latency by promoting the survival of infected neurons. The LAT locus also contains a newly described gene (AL), which is antisense to LAT and partially overlaps LAT encoding sequences. When human (SK-N-SH) or mouse (neuro-2A) neuroblastoma cells were infected with a virus that does not express LAT or AL gene products (dLAT2903), beta interferon (IFN-β) and IFN-α RNA expression was detected earlier relative to the same cells infected with HSV-1 strains that express LAT and AL. Infection of neuro-2A cells with dLAT2903 also led to higher levels of IFN-β promoter activity than in cells infected with wild-type (wt) HSV-1. In contrast, IFN RNA expression was the same when human lung fibroblasts were infected with dLAT2903 or wt HSV-1. When BALB/c mice were infected with dLAT2903, IFN-α and IFN-β RNA expression was readily detected in trigeminal ganglia (TG) 4 days after infection. These transcripts were not detected in TG of mice infected with wt HSV-1 or dLAT2903R (marker-rescued dLAT2903) until 6 days postinfection. When TG single-cell suspensions from infected BALB/c mice were prepared and incubated in vitro with wt HSV-1 as a source of antigen, TG cultures prepared from mice infected with dLAT2903 produced and secreted higher levels of IFN protein than wt HSV-1 or dLAT2903R. Collectively, these studies suggest that the LAT locus interferes with and delays IFN expression.

Original languageEnglish (US)
Pages (from-to)6162-6171
Number of pages10
JournalJournal of virology
Volume79
Issue number10
DOIs
StatePublished - May 1 2005

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Human herpesvirus 1
Trigeminal Ganglion
Human Herpesvirus 1
interferons
Neuroblastoma
Interferons
loci
mice
cells
RNA
neurons
interferon-beta
Neurons
Interferon-beta
Infection
infection
cell suspension culture
Genes
fibroblasts
Suspensions

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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The locus encompassing the latency-associated transcript of herpes simplex virus type 1 interferes with and delays interferon expression in productively infected neuroblastoma cells and trigeminal ganglia of acutely infected mice. / Peng, Weiping; Henderson, Gail; Inman, Melissa; BenMohamed, Lbachir; Perng, Guey Chuen; Wechsler, Steven L.; Jones, Clinton.

In: Journal of virology, Vol. 79, No. 10, 01.05.2005, p. 6162-6171.

Research output: Contribution to journalArticle

Peng, Weiping ; Henderson, Gail ; Inman, Melissa ; BenMohamed, Lbachir ; Perng, Guey Chuen ; Wechsler, Steven L. ; Jones, Clinton. / The locus encompassing the latency-associated transcript of herpes simplex virus type 1 interferes with and delays interferon expression in productively infected neuroblastoma cells and trigeminal ganglia of acutely infected mice. In: Journal of virology. 2005 ; Vol. 79, No. 10. pp. 6162-6171.
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abstract = "The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) is the only abundant viral transcript expressed in latently infected neurons. LAT inhibits apoptosis, suggesting that it regulates latency by promoting the survival of infected neurons. The LAT locus also contains a newly described gene (AL), which is antisense to LAT and partially overlaps LAT encoding sequences. When human (SK-N-SH) or mouse (neuro-2A) neuroblastoma cells were infected with a virus that does not express LAT or AL gene products (dLAT2903), beta interferon (IFN-β) and IFN-α RNA expression was detected earlier relative to the same cells infected with HSV-1 strains that express LAT and AL. Infection of neuro-2A cells with dLAT2903 also led to higher levels of IFN-β promoter activity than in cells infected with wild-type (wt) HSV-1. In contrast, IFN RNA expression was the same when human lung fibroblasts were infected with dLAT2903 or wt HSV-1. When BALB/c mice were infected with dLAT2903, IFN-α and IFN-β RNA expression was readily detected in trigeminal ganglia (TG) 4 days after infection. These transcripts were not detected in TG of mice infected with wt HSV-1 or dLAT2903R (marker-rescued dLAT2903) until 6 days postinfection. When TG single-cell suspensions from infected BALB/c mice were prepared and incubated in vitro with wt HSV-1 as a source of antigen, TG cultures prepared from mice infected with dLAT2903 produced and secreted higher levels of IFN protein than wt HSV-1 or dLAT2903R. Collectively, these studies suggest that the LAT locus interferes with and delays IFN expression.",
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T1 - The locus encompassing the latency-associated transcript of herpes simplex virus type 1 interferes with and delays interferon expression in productively infected neuroblastoma cells and trigeminal ganglia of acutely infected mice

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AU - Perng, Guey Chuen

AU - Wechsler, Steven L.

AU - Jones, Clinton

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