The latency-related gene of bovine herpesvirus 1 inhibits programmed cell death

Janice Ciacci-Zanella, Melissa Stone, Gail Henderson, Clinton J Jones

Research output: Contribution to journalArticle

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Abstract

Although viral gene expression occurs in the peripheral nervous system during acute infection, bovine herpesvirus 1 (BHV-1) gene expression is extinguished, many neurons survive, and latency ensues. The only abundant viral transcript expressed during latency is the latency-related (LR) RNA, which is alternatively spliced in trigeminal ganglia during acute infection (L. Devireddy and C. Jones, J. Virol. 72:7294-7301, 1998). A subset of neurons express a protein encoded by the LR gene and the LR protein (LRP) is associated with cyclin-dependent kinase 2 (Cdk2)/cyclin complexes during productive infection (Y. Jiang, A. Hossain, M. T. Winkler, T. Holt, A. Doster, and C. Jones, J. Virol. 72:8133-8142, 1998). LR gene products inhibit cell cycle progression, perhaps as a result of LRP interacting with Cdk2/cyclin complexes. During acute infection, expression of cyclin A occurs in trigeminal ganglionic neurons (L. M. Schang, A. Hossain, and C. Jones, J. Virol. 70:3807-3814, 1996). Inappropriate expression of G1- and S-phase cyclins can initiate programmed cell death (PCD), apoptosis, in neurons, suggesting that LR gene products inhibit PCD. To test this hypothesis, we modified an assay to measure PCD frequency in transiently transfected cells. C6-ceramide, fumonisin B1 (FB1), or etoposide was used to initiate PCD following transfection of cells with plasmids expressing LR gene products and the β-galactosidase gene. Transfected cells that survived were quantified by counting β-galactosidase-positive cells. Plasmids that expressed LR gene products promoted survival of monkey kidney (CV-1), human lung (IMR-90), or mouse neuroblastoma (neuro-2A) cells after induction of PCD. Plasmids with termination codons at the beginning of LR open reading frames or deletion of sequences that mediate splicing of LR RNA did not promote cell survival following PCD induction. We hypothesize that LR gene products play a role in promoting survival of postmitotic neurons during acute infection or reactivation.

Original languageEnglish (US)
Pages (from-to)9734-9740
Number of pages7
JournalJournal of virology
Volume73
Issue number12
StatePublished - Dec 1 1999

Fingerprint

Bovine Herpesvirus 1
Bovine herpesvirus 1
Cell Death
apoptosis
cyclins
neurons
Cyclins
Neurons
Genes
Galactosidases
Cyclin-Dependent Kinase 2
genes
galactosidases
Infection
plasmids
Plasmids
infection
cyclin-dependent kinase
interphase
cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Ciacci-Zanella, J., Stone, M., Henderson, G., & Jones, C. J. (1999). The latency-related gene of bovine herpesvirus 1 inhibits programmed cell death. Journal of virology, 73(12), 9734-9740.

The latency-related gene of bovine herpesvirus 1 inhibits programmed cell death. / Ciacci-Zanella, Janice; Stone, Melissa; Henderson, Gail; Jones, Clinton J.

In: Journal of virology, Vol. 73, No. 12, 01.12.1999, p. 9734-9740.

Research output: Contribution to journalArticle

Ciacci-Zanella, J, Stone, M, Henderson, G & Jones, CJ 1999, 'The latency-related gene of bovine herpesvirus 1 inhibits programmed cell death', Journal of virology, vol. 73, no. 12, pp. 9734-9740.
Ciacci-Zanella J, Stone M, Henderson G, Jones CJ. The latency-related gene of bovine herpesvirus 1 inhibits programmed cell death. Journal of virology. 1999 Dec 1;73(12):9734-9740.
Ciacci-Zanella, Janice ; Stone, Melissa ; Henderson, Gail ; Jones, Clinton J. / The latency-related gene of bovine herpesvirus 1 inhibits programmed cell death. In: Journal of virology. 1999 ; Vol. 73, No. 12. pp. 9734-9740.
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