The isolation and genetic heterogeneity of Trypanosoma brucei gambiense from north-west Uganda

J. C.K. Enyaru, R. Allingham, T. Bromidge, Georgette D Kanmogne, J. F. Carasco

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Fifty-two samples of blood were taken from sleeping sickness patients in north-west Uganda. All samples failed to infect immunosuppressed mice. Ten cryopreserved blood samples were fed to laboratory bred Glossina morsitans morsitans; eight flies developed midgut infections from which procyclic cultures were established in vitro. Isoenzyme electrophoretic analysis of 9 enzymes revealed that 7 of the 8 trypanosome isolates had a combination of enzyme patterns already described for Trypanosoma brucei gambiense. The eighth isolate had a different aspartate aminotransferase polymorphism which placed it in a new zymodeme. Analysis of polymorphisms in genes for 3 variant surface glycoproteins (VSGs) confirmed that the 8 Ugandan trypanosome isolates were T.b.gambiense and revealed further heterogeneity. The VSG 117 gene was present in all the isolates in a pattern of fragments (equivalent to AnTat 1.8) characteristic for T.b.gambiense. For two other VSG genes characteristic of T.b.gambiense, the LiTat 1.3 gene was present in all the isolates, while the AnTat 11.17 gene was present in only 2 of the 8 isolates.

Original languageEnglish (US)
Pages (from-to)31-39
Number of pages9
JournalActa Tropica
Volume54
Issue number1
DOIs
StatePublished - Jan 1 1993

Fingerprint

Trypanosoma gambiense
Trypanosoma brucei gambiense
Uganda
Genetic Heterogeneity
variant surface glycoproteins
Membrane Glycoproteins
Trypanosomiasis
Genes
genes
genetic polymorphism
Glossina morsitans morsitans
Tsetse Flies
trypanosomiasis
blood
Enzymes
Aspartate Aminotransferases
enzymes
aspartate transaminase
midgut
sampling

Keywords

  • DNA probe
  • Glycoprotein
  • Isoenzyme
  • Trypanosoma brucei gambiense
  • Variable surface glycoprotein (VSG)
  • Zymodeme

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

The isolation and genetic heterogeneity of Trypanosoma brucei gambiense from north-west Uganda. / Enyaru, J. C.K.; Allingham, R.; Bromidge, T.; Kanmogne, Georgette D; Carasco, J. F.

In: Acta Tropica, Vol. 54, No. 1, 01.01.1993, p. 31-39.

Research output: Contribution to journalArticle

Enyaru, J. C.K. ; Allingham, R. ; Bromidge, T. ; Kanmogne, Georgette D ; Carasco, J. F. / The isolation and genetic heterogeneity of Trypanosoma brucei gambiense from north-west Uganda. In: Acta Tropica. 1993 ; Vol. 54, No. 1. pp. 31-39.
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