The intercellular adhesin locus ica is present in clinical isolates of Staphyloccus aureus from bacteremic patients with infected and uninfected prosthetic joints

Vance G. Fowler, Paul D Fey, L. Barth Reller, Anna Lisa Chamis, G. Ralph Corey, Mark Edmund Rupp

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Although polysaccharide intercellular adhesin (PIA) is thought to be crucial in the pathogenesis of prosthetic device infections caused by Staphylococcus epidermidis, its role in prosthetic device infections caused by Staphylococcus aureus is unknown. To assess the clinical impact of PIA production, isolates from 15 prospectively identified cases of S. aureus bacteremia in patients with prosthetic joints (8 infected, 7 uninfected) were characterized for biofilm production, hemagglutination, and the presence of a 419-bp amplification product within icaA. Although icaA was present in all 15 isolates, none of the isolates produced hemagglutination and only one isolate (from a patient with an uninfected prosthetic device) weakly produced biofilm in vitro. These results support the observation that the ica locus is conserved between S. epidermidis and S. aureus and that PIA may be expressed only under in vivo conditions. Future investigations should include animal models to approximate the complex milieu surrounding implanted prosthetic medical devices.

Original languageEnglish (US)
Pages (from-to)127-131
Number of pages5
JournalMedical Microbiology and Immunology
Volume189
Issue number3
DOIs
StatePublished - Dec 1 2000

Fingerprint

Joints
Staphylococcus aureus
Equipment and Supplies
Staphylococcus epidermidis
Hemagglutination
Biofilms
Bacteremia
Infection
Animal Models
polysaccharide intercellular adhesin

Keywords

  • Biofilm
  • Intercellular adhesin locus
  • Polysaccharide intercellular adhesin
  • Prosthetic joint infection
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

Cite this

The intercellular adhesin locus ica is present in clinical isolates of Staphyloccus aureus from bacteremic patients with infected and uninfected prosthetic joints. / Fowler, Vance G.; Fey, Paul D; Reller, L. Barth; Chamis, Anna Lisa; Corey, G. Ralph; Rupp, Mark Edmund.

In: Medical Microbiology and Immunology, Vol. 189, No. 3, 01.12.2000, p. 127-131.

Research output: Contribution to journalArticle

@article{8495c5034c59474b9887ee27141251af,
title = "The intercellular adhesin locus ica is present in clinical isolates of Staphyloccus aureus from bacteremic patients with infected and uninfected prosthetic joints",
abstract = "Although polysaccharide intercellular adhesin (PIA) is thought to be crucial in the pathogenesis of prosthetic device infections caused by Staphylococcus epidermidis, its role in prosthetic device infections caused by Staphylococcus aureus is unknown. To assess the clinical impact of PIA production, isolates from 15 prospectively identified cases of S. aureus bacteremia in patients with prosthetic joints (8 infected, 7 uninfected) were characterized for biofilm production, hemagglutination, and the presence of a 419-bp amplification product within icaA. Although icaA was present in all 15 isolates, none of the isolates produced hemagglutination and only one isolate (from a patient with an uninfected prosthetic device) weakly produced biofilm in vitro. These results support the observation that the ica locus is conserved between S. epidermidis and S. aureus and that PIA may be expressed only under in vivo conditions. Future investigations should include animal models to approximate the complex milieu surrounding implanted prosthetic medical devices.",
keywords = "Biofilm, Intercellular adhesin locus, Polysaccharide intercellular adhesin, Prosthetic joint infection, Staphylococcus aureus",
author = "Fowler, {Vance G.} and Fey, {Paul D} and Reller, {L. Barth} and Chamis, {Anna Lisa} and Corey, {G. Ralph} and Rupp, {Mark Edmund}",
year = "2000",
month = "12",
day = "1",
doi = "10.1007/s430-001-8018-5",
language = "English (US)",
volume = "189",
pages = "127--131",
journal = "Medical Microbiology and Immunology",
issn = "0300-8584",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - The intercellular adhesin locus ica is present in clinical isolates of Staphyloccus aureus from bacteremic patients with infected and uninfected prosthetic joints

AU - Fowler, Vance G.

AU - Fey, Paul D

AU - Reller, L. Barth

AU - Chamis, Anna Lisa

AU - Corey, G. Ralph

AU - Rupp, Mark Edmund

PY - 2000/12/1

Y1 - 2000/12/1

N2 - Although polysaccharide intercellular adhesin (PIA) is thought to be crucial in the pathogenesis of prosthetic device infections caused by Staphylococcus epidermidis, its role in prosthetic device infections caused by Staphylococcus aureus is unknown. To assess the clinical impact of PIA production, isolates from 15 prospectively identified cases of S. aureus bacteremia in patients with prosthetic joints (8 infected, 7 uninfected) were characterized for biofilm production, hemagglutination, and the presence of a 419-bp amplification product within icaA. Although icaA was present in all 15 isolates, none of the isolates produced hemagglutination and only one isolate (from a patient with an uninfected prosthetic device) weakly produced biofilm in vitro. These results support the observation that the ica locus is conserved between S. epidermidis and S. aureus and that PIA may be expressed only under in vivo conditions. Future investigations should include animal models to approximate the complex milieu surrounding implanted prosthetic medical devices.

AB - Although polysaccharide intercellular adhesin (PIA) is thought to be crucial in the pathogenesis of prosthetic device infections caused by Staphylococcus epidermidis, its role in prosthetic device infections caused by Staphylococcus aureus is unknown. To assess the clinical impact of PIA production, isolates from 15 prospectively identified cases of S. aureus bacteremia in patients with prosthetic joints (8 infected, 7 uninfected) were characterized for biofilm production, hemagglutination, and the presence of a 419-bp amplification product within icaA. Although icaA was present in all 15 isolates, none of the isolates produced hemagglutination and only one isolate (from a patient with an uninfected prosthetic device) weakly produced biofilm in vitro. These results support the observation that the ica locus is conserved between S. epidermidis and S. aureus and that PIA may be expressed only under in vivo conditions. Future investigations should include animal models to approximate the complex milieu surrounding implanted prosthetic medical devices.

KW - Biofilm

KW - Intercellular adhesin locus

KW - Polysaccharide intercellular adhesin

KW - Prosthetic joint infection

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=0034450458&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034450458&partnerID=8YFLogxK

U2 - 10.1007/s430-001-8018-5

DO - 10.1007/s430-001-8018-5

M3 - Article

C2 - 11388609

AN - SCOPUS:0034450458

VL - 189

SP - 127

EP - 131

JO - Medical Microbiology and Immunology

JF - Medical Microbiology and Immunology

SN - 0300-8584

IS - 3

ER -